Single-Domain Antibodies : Methods and Protocols / edited by Greg Hussack, Kevin A. Henry.

Format:

Book

Contributor:

Hussack, Greg, Editor.

Henry, Kevin A., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2446

Methods in Molecular Biology, 1940-6029 ; 2446

Language:

English

Subjects (All):

Biology-Technique.

Experimental immunology.

Immunological Techniques.

Local Subjects:

Immunological Techniques.

Physical Description:

1 online resource (XIV, 612 pages) : 95 illustrations, 84 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Single-Domain Antibodies: Methods and Protocols aims to be a useful, practical guide to help researchers further their studies in this field.

Contents:

Nanobodies: From Serendipitous Discovery of Heavy Chain-Only Antibodies in Camelids to a Wide Range of Useful Applications

Overview, Generation, and Significance of Variable New Antigen Receptors (VNARs) as a Platform for Drug and Diagnostic Development

Llama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders

Preparation of Immune and Synthetic VNAR Libraries as Sources of High-Affinity Binders

Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets

A Transgenic Heavy Chain IgG Mouse Platform as a Source of High Affinity Fully Human Single-Domain Antibodies for Therapeutic Applications

Cytoplasmic Production of Nanobodies and Nanobody-Based Reagents by Co-Expression of Sulfhydryl Oxidase and DsbC Isomerase

Small Scale Secretory VHH Expression in Saccharomyces cerevisiae

Production of Single-Domain Antibodies in Pichia pastoris

Production of Designer VHH-Based Antibodies in Plant

Assessing the Aggregation Propensity of Single-Domain Antibodies Upon Heat-Denaturation Employing the ΔTm Shift

Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing

Engineering pH-Sensitive Single-Domain Antibodies

Humanization of Camelid Single-Domain Antibodies

Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues

Introducing Cysteines into Nanobodies for Site-Specific Labelling

Affinity-Guided Site Selective Labelling of Nanobodies With Aldehyde Handles

Cytoplasmic Expression of Nanobodies with Formylglycine Generating Enzyme Tag and Conversion to a Bio-Orthogonal Aldehyde Group

Site-Specific Fluorescent Labelling, Single-Step Immunocytochemistry, and Delivery of Nanobodies into Living Cells

Design and Validation of Site-Specifically Labelled Single-Domain Antibody-Based Tracers for In Vivo Fluorescence Imaging and Image-Guided Surgery.

Design and Preparation of Photobodies: Light-Activated Single-Domain Antibody Fragments

Visualizing Filoviral Nucleoproteins Using Nanobodies Fused to the Ascorbate Peroxidase Derivatives APEX2 and dEAPX

Development of Glypican-2 Targeting Single-Domain Antibody CAR T Cells for Neuroblastoma

Single-Domain Antibodies for Intracellular Toxin Neutralization

Generation of Single-Domain Antibody-Based Recombinant Immunotoxins

X-Ray Crystal Structure Analysis of VHH-Protein Antigen Complexes

Functionalization of Magnetic Beads with Biotinylated Nanobodies for MALDI-TOF/MS-Based Quantitation of Small Analytes

Nanobody-Based Assays for the Detection of Environmental and Agricultural Contaminants

Peptide Tag-Specific Nanobodies for Studying Proteins in Live Cells

Nanobody-Based GFP Traps to Study Protein Localization and Function in Developmental Biology

Optogenetic Activation of Intracellular Nanobodies. .

Other Format:

Printed edition:

ISBN:

978-1-0716-2075-5

9781071620755

Access Restriction:

Restricted for use by site license.