DNA Damage Responses : Methods and Protocols / edited by Nima Mosammaparast.

Format:

Book

Contributor:

Mosammaparast, Nima, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2444

Methods in Molecular Biology, 1940-6029 ; 2444

Language:

English

Subjects (All):

DNA damage.

DNA repair.

DNA Damage and Repair.

Local Subjects:

DNA Damage and Repair.

Physical Description:

1 online resource (XI, 284 pages) : 75 illustrations, 59 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume provides detailed methods and key approaches used to mechanistically study DNA damage, as well as the factors involved in the damage response. Chapters guide readers through proteomics and biophysical approaches, analyzing protein function, quantifying DNA replication dynamics and nucleic acid base damage, as well as biochemical reconstitution of key pathways involved in DNA repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Damage Responses: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Contents:

Robust Computational Approaches to Defining Insights on the Interface of DNA Repair with Replication and Transcription in Cancer

A Whole Genome CRISPR/Cas9 Screening Approach for Identifying Genes Encoding DNA End-Processing Proteins

Immunoaffinity Purification of Epitope-Tagged DNA Repair Complexes from Human Cells

Universally Accessible Structural Data on Macromolecular Conformation, Assembly, and Dynamics by Small Angle X-Ray Scattering for DNA Repair Insights

Assessing DNA damage responses using B lymphocyte cultures

Studying single stranded DNA gaps a­­t replication intermediates by electron microscopy

Approaches to monitor termination of DNA replication using Xenopus egg extracts

Use of high-performance liquid chromatography coupled to mass spectrometry to quantify modified nucleosides

Targeted Formation of 8-oxoguanine in Telomeres

Qualitative and Quantitative Analysis of DNA-Cytidine Deaminase Activity

Characterization of DNA-PK-bound end fragments using GLASS-ChIP

Monitoring Nuclease Activity by X-ray Scattering Interferometry using Gold Nanoparticle Conjugated DNA

In Vitro Reconstitution of BRCA1-BARD1/RAD51 Mediated Homologous DNA Pairing

Purification of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) fromHeLa cells

Purification and characterization of human DNA ligase III complexes after expression in insect cells

Generation of monoubiquitin and K63-linked polyubiquitin chains for protein interaction studies.

Other Format:

Printed edition:

ISBN:

978-1-0716-2063-2

9781071620632

Access Restriction:

Restricted for use by site license.