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Galectins : Methods and Protocols / edited by Sean R. Stowell, Connie M. Arthur, Richard D. Cummings.

SpringerProtocols (1984- current) Available online

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Format:
Book
Contributor:
Stowell, Sean R., Editor.
Arthur, Connie M., Editor.
Cummings, Richard D., Editor.
SpringerLink (Online service)
Series:
Springer Protocols (Springer-12345)
Methods in molecular biology 1940-6029 ; 2442
Methods in Molecular Biology, 1940-6029 ; 2442
Language:
English
Subjects (All):
Life sciences.
Life Sciences.
Local Subjects:
Life Sciences.
Physical Description:
1 online resource (XIX, 730 pages) : 121 illustrations, 86 illustrations in color.
Edition:
2nd ed. 2022.
Contained In:
Springer Nature eBook
Place of Publication:
New York, NY : Springer US : Imprint: Humana, 2022.
System Details:
text file PDF
Summary:
This second edition is dedicated to new and updated methodological approaches designed to study galectin function. Chapters examine salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Galectins: Methods and Protocols, Second Eidtion aims to be a useful practical guide to researches to help further their study in this field. .
Contents:
Galectins: An Ancient Family of Carbohydrate Binding Proteins with Modern Functions
Cloning, Expression and Purification of Galectins for In Vitro Studies
Purification of Recombinant Galectins Using Affinity Chromatography Methods
Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation
Rapid Detection and Purification of Galectin-3 by the Capture and Release (CaRe) Method
Introducing 77Se NMR Spectroscopy to Analyzing Galectin-Ligand Interaction
Evaluation of Galectin Binding by Surface Plasmon Resonance
Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein
Examining Galectin Binding Specificity Using Glycan Microarrays
Mechanism of Mucin Recognition by Lectins - A Thermodynamic Study
Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis
Dissecting Context-Specific Galectin Binding Using Glyco-Engineered Cell Libraries
Method for Identifying Galectin Ligands on Lymphocyte Membrane Glycoproteins
Transformation of Agrocybe Cylindracea Galectin into aGalNAc-specific Lectin
What Happens if a Human Galectin Enters the Endoplasmic Reticulum?
Investigation of Galectins in Frozen Tissue and Mammalian Cell Culture Using Confocal Microscopy
Exploring the Galectin Network by Light and Fluorescence Microscopy
Molecular Imaging for In Vivo Tracking and Detection of Galectin Binding Partners
Visualization of Cytosolic Galectin Accumulation Around Damaged Vesicles and Organelles
Transcytosis of Galectin-3 in Mouse Intestine
Evaluating the Role of Galectins in Clathrin Independent Endocytosis
Examination of Galectin-3-Recruitment into Multivesicular Bodies for Exosomal Secretion
Manipulating Galectin Expression in Zebrafish (Danio Rerio)
Examining Galectin Gene Regulation by Reporter Assays
Examining the Impact of Galectin-9 on Latent HIV Transcription
Evaluation of the Role of Galectins in Parasite Immunity
Evaluation of the Bactericidal Activity of Galectins
Detection of Phosophatidylserine Exposure on Leukocytes Following Treatment with Human Galectins
Detection of Reactive Oxygen Species in Human Neutrophils Under Various Conditions of Exposure to Galectin
Analysis of Galectin-Binding Receptors on B Cells
Methods for Assessing the Effects of Galectins on Leukocytetrafficking and Clearance
Examination of the Contributions of Maternal/Placental-Derived Galectin-1 to Pregnancy Outcome
Method to Study the Role of Galectins in Angiogenesis In Vivo Using the Chick Chorioallantoic Membrane Assay
Untangling Galectin-Mediated Circuits that Control Hypoxia-Driven Angiogenesis
Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology
Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle
Exploring the Role of Galectins in Cancer: In Vitro and In Vivo Approaches.
Other Format:
Printed edition:
ISBN:
978-1-0716-2055-7
9781071620557
Access Restriction:
Restricted for use by site license.

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