Natural Killer (NK) Cells : Methods and Protocols / edited by Noriko Shimasaki.

Format:

Book

Contributor:

Shimasaki, Noriko, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2463

Methods in Molecular Biology, 1940-6029 ; 2463

Language:

English

Subjects (All):

Lymphocytes.

Immunology.

Local Subjects:

Lymphocytes.

Immunology.

Physical Description:

1 online resource (XII, 330 pages) : 50 illustrations, 43 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed book provides a broad collection of methodologies for natural killer (NK) cell research. Beginning with an assortment of methods for the isolation of NK cells and NK cell differentiation, the volume continues with methodologies for functional tests, such as cytotoxicity, viral infection, and metabolism assays, as well as clinical applications of NK cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Natural Killer (NK) Cells: Methods and Protocols serves as an ideal guide for researchers looking to contribute to the further development of basic and clinical NK cell research.

Contents:

Isolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria

Purification of Primary Decidual Natural Killer Cells for Functional Analysis

In Vitro Development of Mouse and Human NK Cells from Hematopoietic Progenitor Cells

Induction of Human Natural Killer Cells under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells

Development of Humanized Mouse Models for Studying Human NK Cells in Health and Disease

Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10x Genomics' Single-Cell RNA-Seq

Methods to Analyze the Developmental Trajectory of Human Primary NK Cells Using Monocle and SCENIC Analyses

Methods for Isolating and Defining Single-Cell Transcriptomes of Tissue-Resident Human NK Cells

Retrogenic Color-Barcoding for Fate Mapping of Single Innate Lymphocytes

Quantifying Human Natural Killer Cell Migration by Imaging and Image Analysis

Tracking of NK Cells by Positron Emission Tomography Using 89Zr-Oxine Ex Vivo Cell Labeling

Assessing Changes in Human Natural Killer Cell Metabolism Using the Seahorse Extracellular Flux Analyzer

Determining Activation Status of Natural Killer Cells Following Stimulation via Cytokines and Surface Receptors

Method to Study Adaptive NK Cells Following MCMV Infections

Assessing the Response of Human NK Cell Subsets to Infection by Clinically-Isolated Virus Strains

NK Cell Isolation and Cytotoxicity by Radioactive Chromium Release Assay and DELFIA -EuTDA Cytotoxicity Assay

Organoid Co-Culture Methods to Capture Cancer Cell -Natural Killer Cell Interactions

In Vitro Visualization of Cell-to-Cell Interactions between Natural Killer Cells and Sensory Neurons

CRISPR Screen to Identify Factors that Render Tumor Cells Sensitive or Resistant to Killing by NK Cells

Practical Considerations and Workflow in Utilizing KIR Genotyping in Transplantation Medicine

Gene Transduction of Natural Killer Cells for Clinical Application.

Other Format:

Printed edition:

ISBN:

978-1-0716-2160-8

9781071621608

Access Restriction:

Restricted for use by site license.