Plasmodesmata : Methods and Protocols / edited by Yoselin Benitez-Alfonso, Manfred Heinlein.

Format:

Book

Contributor:

Benitez-Alfonso, Yoselin, Editor.

Heinlein, Manfred, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2457

Methods in Molecular Biology, 1940-6029 ; 2457

Language:

English

Subjects (All):

Plants.

Plant cells and tissues.

Botany.

Plant Signalling.

Plant Cell Biology.

Plant Science.

Local Subjects:

Plant Signalling.

Plant Cell Biology.

Plant Science.

Physical Description:

1 online resource (XIII, 470 pages) : 90 illustrations, 87 illustrations in color.

Edition:

2nd ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plasmodesmata: Methods and Protocols, Second Edition serves as a vital guide for all plant scientists, both novice and expert, especially those studying the intricacies of cell-to-cell communication pathways. .

Contents:

Plasmodesmata Structural Components and Their Role in Signaling and Plant Development

Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses

Plasmodesmata Ultrastructure Determination Using Electron Tomography

Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata

Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots

Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities

Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy

Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy

In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata

Immunofluorescence Detection of Callose in Plant Tissue Sections

Callose Detection and Quantification at Plasmodesmata in Bryophytes

Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis

Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons

Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer

Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy

Using Steady-State Fluorescence Anisotropy to Study Protein Clustering

Quantification of Cell-to-Cell Connectivity Using Particle Bombardment

Investigating Plasmodesmata Function in Arabidopsis thaliana Using a Low-Pressure Bombardment System and GFP Movement Assay

Quantifying Plasmodesmatal Transport with an Improved GFP Movement Assay

An Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins

Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP

Tracking Intercellular Movement of Fluorescent Proteins in Bryophytes

Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability

Analysis of the Distribution of Symplasmic Tracers during Zygotic and Somatic Embryogenesis

Quantifying Intercellular Movement and Protein Stoichiometry for Computational Modeling

Spatiotemporal Specific Blocking of Plasmodesmata by Callose Induction

A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue

In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG

Multi-Angle In Vivo Imaging of the Arabidopsis thaliana Shoot Apical Meristem (SAM)

More Insights from Ultrastructural and Functional Plasmodesmata Data Using PDinsight

Measuring Intercellular Interface Area in Plant Tissues Using Quantitative 3D Image Analysis.

Other Format:

Printed edition:

ISBN:

978-1-0716-2132-5

9781071621325

Access Restriction:

Restricted for use by site license.