Whole-Body Regeneration : Methods and Protocols / edited by Simon Blanchoud, Brigitte Galliot.

Format:

Book

Contributor:

Blanchoud, Simon, Editor.

Galliot, Brigitte, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2450

Methods in Molecular Biology, 1940-6029 ; 2450

Language:

English

Subjects (All):

Regenerative medicine.

Stem cells.

Regenerative Medicine and Tissue Engineering.

Stem Cell Biology.

Local Subjects:

Regenerative Medicine and Tissue Engineering.

Stem Cell Biology.

Physical Description:

1 online resource (XVII, 685 pages) : 131 illustrations, 123 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing on the primary challenges of this research. Parts Two and Three explore a series of non-vertebrate zoological contexts that provide experimental models for WBR, showing how they can be approached with cellular tools. Parts Four, Five, and Six discuss the future advancements of WBR, reporting about the cutting-edge techniques in genetics and omics used to dissect the underlying mechanisms of WBR, and systems biology approaches to reach a synthetic view of WBR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Whole-Body Regeneration: Methods and Protocols is a valuable resource for scientists and researchers who want to learn more about this important and developing field.

Contents:

The Hazards of Regeneration: From Morgan's Legacy to Evo-Devo

Studying Regeneration in Ascidians: An Historical Overview

Studying Protista WBR and Repair using Physarum polycephalum

Studying Porifera WBR using the Calcerous Sponges Leucosolenia

Studying Ctenophora WBR using Mnemiopsis leidyi

Studying Placozoa WBR in the Simplest Metazoan Animal, Trichoplax adhaerens

Collecting and Culturing Kamptozoans for Regenerative Studies

Collecting and Culturing Bryozoans for Regenerative Studies

Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride

Studying Annelida Body Regeneration under Environmental Stress in Diopatra neapolitana

Studying Annelida Regeneration using Platynereis dumerilii

Collecting and Culturing Lineus sanguineus to Study Nemertea WBR

Studying Xenacoelomorpha WBR using Isodiametra pulchra

Studying Echinodermata Arm Explant Regeneration using Echinaster sepositus

Studying Hemichordata WBR using Ptychodera flava

Studying Tunicata WBR using Botrylloides anceps

In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis

Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi

Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals

Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry

Non-Invasive Intra-Vascular Micro-Transfusion in Colonial Tunicates

Gene Manipulation in Hydractinia

Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensis

Monitoring Telomere Maintenance during Regeneration of Annelids

Analysis of DNA Double Stranded Breaks using the COMET Assay in Planarians

Random Integration Transgenesis in a Free-Living Regenerative Flatworm Macrostomum lignano

RNAi Screening to Assess Tissue Regeneration in Planarians

Monitoring Chromatin Regulation in Planarians using Chromatin Immunoprecipitation followed by Sequencing (ChIP-seq)

Assessing Chromatin Accessibility during WBR in Acoels

Single Cell Transcriptomic Analysis in the Regenerating Cnidarian Nematostella vectensis

Characterization of Soluble Cell-Free Coelomic Fluid Proteome from the Starfish Marthasterias glacialis

Using RNA-Sequencing for Transcriptome Profiling of Botrylloides sp. Regeneration

Studying Mechanical Oscillations during Whole-Body Regeneration in Hydra

Combining RNAi-Mediated β-catenin Inhibition and Reaggregation to Study Hydra Whole-Body Regeneration

Creating a User-Friendly and Open-Access Gene Expression Database for Comparing Embryonic Development and Regeneration in Nematostelle vectensis

Formalizing Phenotypes of Regeneration.

Other Format:

Printed edition:

ISBN:

978-1-0716-2172-1

9781071621721

Access Restriction:

Restricted for use by site license.