Axonal Transport : Methods and Protocols / edited by Alessio Vagnoni.

Format:

Book

Contributor:

Vagnoni, Alessio, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2431

Methods in Molecular Biology, 1940-6029 ; 2431

Language:

English

Subjects (All):

Zoology.

Neurosciences.

Neuroscience.

Local Subjects:

Zoology.

Neuroscience.

Physical Description:

1 online resource (XV, 573 pages) : 145 illustrations, 126 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume covers a wide range of model systems from invertebrate to humans and in vitro and in vivo. Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Axonal Transport: Methods and Protocols aims to further our understanding of the trafficking of cellular components in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of the neuron. The chapters " Live imaging of RNA Transport and Translation in Xenopus Retinal Axons, Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons, Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons, Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport, and In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport" are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

The Giant Axon of the Squid: A Simple System for Axonal Transport Studies

Live Imaging and Quantitative Analysis of Organelle Transport In Sensory Neurons of Aplysia Californica

Live imaging of RNA Transport and Translation in Xenopus Retinal Axons

Imaging Axonal Transport in Ex Vivo Central and Peripheral Nerves

In vivo Imaging of Axonal Organelle Transport in the Mouse Brain

Studying Axonal Transport in the Brain by Manganese-Enhanced Magnetic Resonance Imaging (MEMRI)

Microfluidic Neuromuscular Co-Culture System for Tracking Cell-To-Cell Transfer and Axonal Transport of Labeled Proteins

Imaging Diversity in Slow Axonal Transport

Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses

Molecular Analysis of Axonal Transport Dynamics Upon Modulation of Microtubule Acetylation

Live-cell Imaging of RNA Transport In Axons Of Cultured Primary Neurons

Visualizing Vesicle-Bound Kinesins in Cultured Hippocampal Neurons

Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons

Use of Microfluidics Chambers to Image Axonal Transport In Adult Sensory Neurons

High-resolution Imaging of Mitochondria and Mitochondrial Nucleoids in Differentiated SH-SY5Y Cells

Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons

In vivo Live Imaging of Axonal Transport In Developing Zebrafish Axons

Visualizing the Intracellular Trafficking in Zebrafish Mauthner Cells

Dissection and Direct Imaging of Axonal Transport in Drosophila Segmental Nerves

Detailed Imaging of Mitochondrial Transport and Precise Manipulation of Mitochondrial Function with Genetically-Encoded Photosensitizers in Adult Drosophila Neurons

Mitochondrial DNA Transport in Drosophila neurons

Live imaging of Axonal Transport in the Adult Drosophila Central Nervous System

Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport

High-resolution Live-Imaging of Axonal RNP granules in Drosophila Pupal Brain Explants

Analyzing the Impact of Gene Mutations on Axonal Transport in Caenorhabditis elegans

Methods to Quantify and Relate Axonal Transport Defects to Changes In C. Elegans Behavior

Imaging Intracellular Trafficking in Neurons of C. elegans

In vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport

In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport.

Other Format:

Printed edition:

ISBN:

978-1-0716-1990-2

9781071619902

Access Restriction:

Restricted for use by site license.