The Nuclear Pore Complex : Methods and Protocols / edited by Martin W. Goldberg.

Format:

Book

Contributor:

Goldberg, Martin W., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2502

Methods in Molecular Biology, 1940-6029 ; 2502

Language:

English

Subjects (All):

Cytology.

Biomolecules.

Physical biochemistry.

Macromolecules.

Cell Biology.

Structural Biology.

Local Subjects:

Cell Biology.

Structural Biology.

Physical Description:

1 online resource (XIV, 515 pages) : 100 illustrations, 76 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume covers all methods used to discover the composition, structure, and dynamics of the nuclear pore complex (NPC), as well as the soluble transport factors involved in the transport process. Chapters detail structural biology methods, biophysical, transport factors , and a wide range of model organisms that have been used over the years. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, The Nuclear Pore Complex: Methods and Protocols: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .

Contents:

Affinity Isolation of Endogenous Saccharomyces cerevisiae Nuclear Pore Complexes

Transformation of Chaetomium thermophilum and affinity-purification of native thermostable protein complexes

Nuclear Pore Complex Assembly using Xenopus Egg Extract

Analysis of Nuclear Pore Complex Permeability in Mammalian Cells and Isolated Nuclei Using Fluorescent Dextrans

Hormone-inducible Transport Reporter Assay to Study Nuclear Import Defects in Neurodegenerative Diseases

Subcellular Fractionation Suitable for Studies of RNA and Protein Trafficking

Localising Total mRNA in Plant Cells

Using Single Molecule RNA FISH to Determine Nuclear Export and Transcription Phenotypes in Drosophila Tissues

Analysis of Nucleoporin Function using Inducible Degron Techniques

Monitoring of Chromatin Organization at the Nuclear Pore Complex, Inner Nuclear Membrane and Nuclear Interior in Live Cells by Fluorescence Ratiometric Imaging of Chromatin (FRIC)

Analysis of Nuclear Pore Complexes in Caenorhabditis elegans by Live Imaging and Functional Genomic

Protein Retargeting in Aspergillus nidulans to Study the Function of Nuclear Pore Complex Proteins

Split-GFP Complementation to Study the Nuclear Membrane Proteome using Microscopy

Bimolecular Fluorescence Complementation: Quantitative Analysis of In Cell Interaction of Nuclear Transporter Importin a with Cargo Protein

Validation of Nuclear Pore Complex Protein-Protein Interactions by Transient Expression in Plants

Binding affinity measurement of Nuclear Export Signal peptides to their exporter CRM1

Analysis of Ubiquitylation and SUMOylation of Yeast Nuclear Pore Complex Proteins

Purification of Cdk-CyclinB-Kinase-Targeted Phosphopeptides from Nuclear Envelope

Crystallization of Nuclear Export Signals or Small Molecule Inhibitors Bound to Nuclear Exporter CRM1

Atomic Force Microscopy for Structural and Biophysical Investigations on Nuclear Pore Complexes

Multivalent Interactions with Intrinsically Disordered Proteins Measured by Surface Plasmon Resonance

Assembly and Use of a Microfluidic Device to Study Nuclear Mechanobiology during Confined Migration

SPEED Microscopy: High-Speed Single-Molecule Tracking and Mapping of Nucleocytoplasmic Transport

Imaging Fluorescent Nuclear Pore Complex Proteins in C. elegans

Visualizing Nuclear Pore Complexes in Xenopus Egg Extracts

TEM Imaging of Membrane Choreography During Mitosis of Drosophila Tissue Culture Cells

Scanning Electron Microscopy (SEM) and Immuno-SEM of Nuclear Pore Complexes from Amphibian Oocytes, Mammalian Cell Cultures, Yeast and Plants

NPC Structure in Model Organisms: Transmission Electron Microscopy and Immuno-gold Labelling using High Pressure Freezing/Freeze Substitution of Yeast, Worms and Plants

High Resolution Imaging and Analysis of Individual Nuclear Pore Complexes

Live CLEM imaging of Tetrahymena to Analyze the Dynamic Behavior of the Nuclear Pore Complex

Visualizing Nuclear Pore Complex Assembly In Situ in Human Cells at Nanometer Resolution by Correlating Live Imaging with Electron Microscopy.

Other Format:

Printed edition:

ISBN:

978-1-0716-2337-4

9781071623374

Access Restriction:

Restricted for use by site license.