Plant Gametogenesis : Methods and Protocols / edited by Christophe Lambing.

Format:

Book

Contributor:

Lambing, Christophe, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2484

Methods in Molecular Biology, 1940-6029 ; 2484

Language:

English

Subjects (All):

Botany.

Plant genetics.

Bioinformatics.

Plant Science.

Plant Genetics.

Local Subjects:

Plant Science.

Plant Genetics.

Bioinformatics.

Physical Description:

1 online resource (XIV, 382 pages) : 93 illustrations, 83 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume provides protocols and methods on techniques to study plant gametogenesis. Chapters are divided into four sections covering omics, cytological, molecular approaches, plant transformation, genome editing, bioinformatics, and data analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Gametogenesis: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

Contents:

Measurement of flower metabolite concentrations using Gas-Chromatography Mass Spectrometry and High-Performance Liquid Chromatography Mass Spectrometry

Proteomic-, phosphoproteomic- and Acetylomic-based mass spectrometry to identify tissue-specific protein complexes and phosphorylation in plant gametogenesis

Identifying small RNAs and analyzing their association with gene expression using isolated Arabidopsis male meiocytes

Genotyping-by-sequencing to map meiotic crossovers in plants

Interrogating global chromatin interaction network by high-throughput chromosome conformation capture (Hi-C) in plants

Cytogenetic techniques for analysing meiosis in hexaploidy bread wheat

Super-resolution chromatin visualisation by combined fluorescence in situ hybridisation and Structured Illumination Microscopy in Solanum lycopersicum

Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy

Bimolecular Fluorescence Complementation to Test for Protein-Protein Interactions and To Uncover Regulatory Mechanisms During Gametogenesis

Quantifying meiotic crossover recombination in Arabidopsis lines expressing fluorescent reporters in seeds using SeedScoring pipeline for CellProfile

Generation of mutants by combined treatment of physical and chemical mutagens in rice

Generation of mutant plants by Gamma Ray exposure and development of low-cost TILLING population in chickpea (Cicer arietinum L.)

Efficient generation of CRISPR/Cas9-based mutants supported by fluorescent seed selection in different Arabidopsis accessions

Generation of deletion lines in Allohexaploid Bread Wheat

Post-transcriptional Gene Silencing Using Virus-Induced Gene Silencing to Study Plant Gametogenesis in Tomato

Protocols for in vivo doubled haploid (DH) technology in maize breeding: From haploid inducer Development to Haploid Genome Doubling

Estimation of heterografting associated DNA methylation changes in tree crops by MSAP analysis

In vitro fertilization system using wheat gametes by electric fusion

High-throughput fluorescent pollen tetrad analysis using DeepTetrad

Barley (Hordeum vulgare) anther and meiocyte RNA Sequencing: Mapping Sequencing reads and downstream data analyses

Profiling m6A RNA modifications in low amounts of plant cells using Maize Meiocytes

Assembling Plant Genomes with Long-Read Sequencing

Mapping-by-sequencing of point and insertional mutations with Easymap

Characterization of the different levels of variation in 45S rRNA genes.

Other Format:

Printed edition:

ISBN:

978-1-0716-2253-7

9781071622537

Access Restriction:

Restricted for use by site license.