Microtubules : Methods and Protocols / edited by Hiroshi Inaba.

Format:

Book

Contributor:

Inaba, Hiroshi, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2430

Methods in Molecular Biology, 1940-6029 ; 2430

Language:

English

Subjects (All):

Biomedical engineering.

Biomedical Engineering and Bioengineering.

Local Subjects:

Biomedical Engineering and Bioengineering.

Physical Description:

1 online resource (XIV, 486 pages) : 131 illustrations, 110 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume provides state-of-the-art methodology to functionalize microtubules and construct microtubule-based materials. Chapters consists of six sections detailing modification of microtubule structures, observation and control of microtubule movement, material applications of microtubules in vitro, development of microtubule-binding molecules, comprehensive approaches to analyze properties of microtubules, and functionalization of microtubules in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microtubules: Methods and Protocols aims to provide useful information for construction of microtubule-based materials and help to create new ideas for the next-generation of materials and applications through detailed protocols.

Contents:

Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modification

Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule Associated Intracellular Function

Functionalization of Tubulin: Approaches to Modify Tubulin with Biotin and DNA

Electro-Modulation of Tubulin Properties and Function

In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference Reflection Microscopy

Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay using FLIC Microscopy

Design of Mechanical and Electrical Properties for Multi-Directional Control of Microtubules

Linear-Zero Mode Waveguides for Single-Molecule Fluorescence Observation of Nucleotides in Kinesin-Microtubule Motility Assay

Microtubules and Quantum Dots Integration Leads to Conjugates with Applications in Biosensors and Bionanodevices

Assembling Microtubule-Based Active Matter

Spontaneous Alignment of Microtubules via Tubulin Polymerization in a Narrow Space under a Temperature Gradient

Dynamic Pattern Formation of Active Matters Triggered by Mechanical Stimuli

Spontaneously Beating Biomimetic Structures

Construction of Molecular Robots from Microtubules for Programmable Swarming

Fabrication of Artificial Muscle from Microtubules, Kinesins and DNA Origami Nanostructures

Encapsulation of Nanomaterials Inside Microtubules by Using a Tau-derived Peptide

Investigating Tubulin-Drug Interaction Using Fluorescence Spectroscopy

Visualization and Quantification of Microtubule Self-repair

Cargo Transport by Microtubule-Associated Motor Protein Along Mechanically Deformed Microtubules

Mechanical Deformation of Microtubules on a Two-Dimensional Elastic Medium

Real-Time Imaging of Single γTuRC-Mediated Microtubule Nucleation Events In Vitro by TIRF Microscopy

Microtubule Preparation for Investigation with High-Speed Atomic Force Microscopy

Crystallization Systems for the High-Resolution Structural Analysis of Tubulin-Ligand Complexes

Cryo-EM Visualization of Neuronal Particles Inside Microtubules

Reconstituting the Interaction Between Purified Nuclei and Microtubule Network

Photocontrolling Microtubule Dynamics with Photoswitchable Chemical Reagents

Monitoring the Disruptive Effects of Tubulin Binding Agents on Cellular Microtubules

Pacific Blue-Taxoids as Fluorescent Molecular Probes of Microtubules

Controlling Cell Shape and Microtubule Organization by Extracellular Matrix Micropatterning. .

Other Format:

Printed edition:

ISBN:

978-1-0716-1983-4

9781071619834

Access Restriction:

Restricted for use by site license.