Yeast Surface Display / edited by Michael W. Traxlmayr.

Format:

Book

Contributor:

Traxlmayr, Michael W., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2491

Methods in Molecular Biology, 1940-6029 ; 2491

Language:

English

Subjects (All):

Proteins.

Protein Biochemistry.

Local Subjects:

Protein Biochemistry.

Physical Description:

1 online resource (XV, 646 pages) : 109 illustrations, 96 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.

Contents:

Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System

Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation

Site-Wise Diversification of Combinatorial Libraries Using Insights from Structure-Guided Stability Calculations

Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution

Machine Learning-Driven Protein Library Design: A Path Toward Smarter Libraries

Kinetic Competition Screening of Yeast-Displayed Libraries for the Generation of High Affinity Binders

Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display

Engineering Binders with Exceptional Selectivity

Affinity and Stability Analysis of Yeast Displayed Proteins

Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting

A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins

Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques

Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display

Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology

Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors

Yeast Display Guided Selection of pH Dependent Binders

Yeast Mating as a Tool for Highly Effective Discovery and Engineering of Antibodies via Display Methodologies

Humanization of Chicken-Derived Antibodies by Yeast Surface Display

Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display

Discovery of Cyclic Peptide Binders from Chemically Constrained Yeast Display Libraries

Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays

Isolating Anti-Amyloid Antibodies from Yeast-Displayed Libraries

Engineering Proteins Containing Noncanonical Amino Acids on the Yeast Surface

Construction of Yeast Display Libraries for Selection of Antigen-Binding Variants of Large Extracellular Loop of CD81, a Major Surface Marker Protein of Extracellular Vesicles

Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display

Simultaneous Display of Multiple Kinds of Enzymes on the Yeast Cell Surface for Multistep Reactions.

Other Format:

Printed edition:

ISBN:

978-1-0716-2285-8

9781071622858

Access Restriction:

Restricted for use by site license.