Epiblast Stem Cells : Methods and Protocols / edited by Pierre Osteil.

Format:

Book

Contributor:

Osteil, Pierre, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2490

Methods in Molecular Biology, 1940-6029 ; 2490

Language:

English

Subjects (All):

Stem cells.

Developmental biology.

Stem Cell Biology.

Developmental Biology and Stem Cells.

Local Subjects:

Stem Cell Biology.

Developmental Biology and Stem Cells.

Physical Description:

1 online resource (XI, 303 pages) : 71 illustrations, 62 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This book aims to deliver the most up-to-date protocols in using epiblast stem cells (EpiSC) to answer critical questions on mammalian development. Beginning with a section on the spectrum of mouse primed pluripotent stem cells, the volume continues with chapters on toolkits to characterize and study primed stem cells, as well as primed stem cells to engineer gastrulation models. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epiblast Stem Cells: Methods and Protocols serves as an ideal guide to overcome the reproducibility problem by gathering detailed state-of-the-art protocols to perform comprehensive analyses, enabling the reader to opt for the appropriate cell type to model the epiblast.

Contents:

Establishment of Mouse Epiblast Stem Cells

L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell

Generation of Epiblast-Like Cells

Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence

Small Interfering RNA (siRNA) Transfection in Epiblast Stem Cells

Measuring Endocytosis and Endosomal Uptake at Single Cell Resolution

3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells

Flow Cytometric Characterization of Pluripotent Cell Protein Markers in Naïve, Formative, and Primed Pluripotent Stem Cells

Exploring Chromatin Accessibility in Mouse Epiblast Stem Cells with ATAC-Seq

A Reproducible and Dynamic Workflow for Analysis and Annotation of scRNA-Seq Data

Complete Transcriptome Analysis by 5'-End Single-Cell RNA-Seq with Random Priming

Global Proteomic Profiling of Embryonic Stem Cells Using iTRAQ Isobaric Tags with LC-MS/MS Quantification

Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-Like Cells

Generation of Gastruloids from Epiblast-Like Cells

Definitive Endoderm from EpiSC Aggregates in Matrigel

In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-Like Cells (PGCLCs)

Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Human Primordial Germ Cell-Like Cells (hPGCLCs) In Vitro

Differentiation of EpiLCs on Micro-Patterned Substrates Generated by Micro-Contact Printing

Grafting of Epiblast Stem Cell into the Epiblast and Whole-Embryo Imaging to Unveil Lineage Competence

Modeling Epiblast Shape in Implanting Mammalian Embryos.

Other Format:

Printed edition:

ISBN:

978-1-0716-2281-0

9781071622810

Access Restriction:

Restricted for use by site license.