Enhancers and Promoters : Methods and Protocols / edited by Tilman Borggrefe, Benedetto Daniele Giaimo.

Format:

Book

Contributor:

Borggrefe, Tilman, Editor.

Giaimo, Benedetto Daniele, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2351

Methods in Molecular Biology, 1940-6029 ; 2351

Language:

English

Subjects (All):

Genetics.

Medical genetics.

Genetics and Genomics.

Medical Genetics.

Local Subjects:

Genetics and Genomics.

Medical Genetics.

Physical Description:

1 online resource (XI, 376 pages) : 69 illustrations, 57 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays. Written in the Methods in Molecular Biology series format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting. Authoritative and cutting-edge, Enhancers and Promoters: Methods and Protocols is a useful guide for future experiments. Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

A New Toolbox to Analyze Enhancer-Promoter Functions

Global Run-on Sequencing (GRO-Seq)

Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET-Seq)

Low Quantity single strand CAGE (LQ-ssCAGE) Maps Regulatory Enhancers and Promoters

Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase-Seq

Measuring Chromatin Accessibility: ATAC-Seq

High-resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles

Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes

Low Input Targeted Chromatin Capture (low-T2C)

Proximity Ligation-assisted ChIP-Seq (PLAC-Seq)

Analysis of Enhancer-promoter Interactions using CAGE and RADICL-Seq technologies

Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to identify open chromatin interactions

Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C)

Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-Translational Modification (PTM) Combinatorics At Distinct Genomic Regions

Using ChIP-SICAP to Identify Proteins That Co-Localize in Chromatin

Genome-wide profiling of protein-DNA Interactions with Chromatin Endogenous Cleavage And High-Throughput Sequencing (ChEC-Seq)

Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators

Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors

Simultaneous Tagmentation-based detection of ChIP/ATAC Signal with Bisulfite Sequencing

Low Input Whole-Genome Bisulfite Sequencing.

Other Format:

Printed edition:

ISBN:

978-1-0716-1597-3

9781071615973

Access Restriction:

Restricted for use by site license.