Glycosylation : Methods and Protocols / edited by Gavin P. Davey.

Format:

Book

Contributor:

Davey, Gavin P., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2370

Methods in Molecular Biology, 1940-6029 ; 2370

Language:

English

Subjects (All):

Biotechnology.

Biochemistry.

Bioinformatics.

Local Subjects:

Biotechnology.

Biochemistry.

Bioinformatics.

Physical Description:

1 online resource (XI, 332 pages) : 113 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume explores the latest techniques and methods used for performing up-to-date glycosylation research. The chapters in this book are organized into four parts. Part One looks at the latest analytical and bioinformatics technologies that enable the characterization of glycosylation complexity. Part Two details the importance of synthetic chemistry and glycoengineering in the fields of bioprocessing and biotherapeutic development. Part Three discusses systems biology and computational technologies used by scientists to analyze glycosylation events in the cell. Part Four focuses on how cellular glycosylation biomarkers can be identified and used to characterize human clinical datasets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Glycosylation: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field. .

Contents:

Glycoinformatics Tools for Comprehensive Characterization of Glycans Enzymatically Released from Proteins

GlycoStore: A Platform for H/UPLC and Capillary Electrophoresis Glycan Data

An Interactive View of Glycosylation

Characterization and Analysis of Food-Sourced Carbohydrates

Comparative Glycomics Analysis of Mass Spectrometry Data

Cell Free Remodeling of Glycosylation of Antibodies

Rapid Antibody Glycoengineering in CHO Cells via RNA Interference and CGE-LIF N-Glycomics

In Silico Analysis of Therapeutic Antibody Aggregation and the Influence of Glycosylation

Recent Advances Towards Engineering Glycoproteins using Modified Yeast Display Platforms

Computational Modeling of Glycan Processing in the Golgi for Investigating Changes in the Arrangements of Biosynthetic Enzymes

O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks

Synthetic Strategies for FRET-Enabled Carbohydrate Active Enzyme Probes

N-Glycan Characterization by Liquid Chromatography Coupled with Fluorimetry and Mass Spectrometry

Lectin Histochemistry for Tissues and Cells, and Dual Lectin and Antibody Co-Localization

Lectin Affinity Chromatography for the Discovery of Novel Cancer Glycobiomarkers: A Case Study with PSA Glycoforms and Prostate Cancer

Metabolic Labelling of Primary Neurons using Carbohydrate Click-Chemistry

Monitoring the Sialome on Human Immune Cells.

Other Format:

Printed edition:

ISBN:

978-1-0716-1685-7

9781071616857

Access Restriction:

Restricted for use by site license.