Glycosaminoglycans : Methods and Protocols / edited by Kuberan Balagurunathan, Hiroshi Nakato, Umesh Desai, Yukio Saijoh.

Format:

Book

Contributor:

Balagurunathan, Kuberan, Editor.

Nakato, Hiroshi, Editor.

Desai, Umesh., Editor.

Saijoh, Yukio, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2303

Methods in Molecular Biology, 1940-6029 ; 2303

Language:

English

Subjects (All):

Biochemistry.

Proteins.

Local Subjects:

Biochemistry.

Proteins.

Physical Description:

1 online resource (XXI, 829 pages) : 180 illustrations, 114 illustrations in color.

Edition:

2nd ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Glycosaminoglycans: Methods and Protocols, Second Edition is a valuable experimental manual for practicing researchers including graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs. .

Contents:

One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan

Solution NMR Spectroscopy for Characterizing Protein-Glycosaminoglycan Interactions

Metabolic Labeling of Proteoglycans and Analysis of Their Synthesis and Sorting in Filter-Grown and Polarized Epithelial Cells

Fluorous-Tag Assisted Synthesis of GAG-Like Oligosaccharides

Aqueous Molecular Dynamics for Understanding Glycosaminoglycan Recognition by Proteins

A Non-Radioactive Method to Measure Hyaluronan Synthase Activity

A Glycoproteomic Approach to Identify Novel Proteoglycans

Analysis of Protein-Glycosaminoglycan Interactions using Traveling Wave Ion Mobility Mass Spectrometry

Ascidian (Chordata-Tunicata) Glycosaminoglycans: Extraction, Purification, Biochemical, and Spectroscopic Analysis

Quantitative Disaccharide Profiling of Glycosaminoglycans from Two Different Preparations by PMP and Deuterated PMP Labeling

Preparation and Characterization of Heparan Sulfate Derived Oligosaccharides to Investigate Protein-GAG Interaction and HS Biosynthesis Enzyme Activity

Heparan Sulfate Structure: Methods to Study N-sulfation and NDST Action

Methods for the Production of Recombinant Heparosan: A Critical Heparin Precursor, from Non-Pathogenic E.coli Strains

De novo Sequencing of Heparin/Heparan Sulfate Oligosaccharides by Chemical Derivatization and LC-MS/MS

Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans

Nuclear Magnetic Resonance Methods in Structural Characterization of Glycosaminoglycans

Aggrecan: Approaches to Study Biophysical and Biomechanical Properties

Chromatographic Molecular Weight Measurements for Heparin: Its Fragments and Fractions, and Other Glycosaminoglycans

Mass Spectrometric Methods for the Analysis of Heparin and Heparan Sulfate

Affinity Purification of Glycosylphosphatidylinositol-Anchored Proteins by Alpha-Toxin

Thermodynamic Affinity and Nature of Forces Defining Glycosaminoglycan: Protein Systems Using Fluorescence Spectroscopy

Isolation and Characterization of Heparan Sulfate Containing Amyloid Precursor Protein Degradation Products

Chemical Approaches to Prepare Modified Heparin and Heparosan Polymers for Biological Studies

Glycosaminoglycan Quality Control by Monosaccharide Analysis

Characterizing Thermodynamics of Protein-Glycosaminoglycan Interactions using Isothermal Titration Calorimetry

Automated Synthesis of Chondroitin Sulfate Oligosaccharides

Capillary Electrophoretic Analysis of Isolated Sulfated Polysaccharides to Characterize Pharmaceutical Products

Preparation of Isotope Enriched Heparan Sulfate Precursors for Structural Biology Studies

Methods for Measuring Exchangeable Protons in Glycosaminoglycans

Advances in Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis

anexVis: A Transcriptome Tool to Visualize Organ/Tissue-Specific Glycosaminoglycan Biosynthetic and Catabolic Pathways in Human Health and Diseases

Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila

Measuring Sulfatase Expression and Invasion in Glioblastoma

Analyzing the Role of Heparan Sulfate Proteoglycans in Axon Guidance In Vivo in Zebrafish

Regulation of 3-O-Sulfation of Heparan Sulfate during Transition from the Naïve to the Primed State in Mouse Embryonic Stem Cells

Analysis of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility in Myofibroblast Differentiation

Methods to Analyze the Effect of Diet-Derived Metabolites on Endothelial Inflammation and Cell Surface Glycosaminoglycans

Assays for Evaluation of Substrates for and Inhibitors of β-1,4-Galactosyltransferase 7

Enzymatic Alteration of ECM to Explore Muscle Function and Motor Control of a Learned Behavior

Procedures to Evaluate the Role of Heparan Sulfate in the Reactivity of Resistance and Conductance Arteries Ex Vivo

Computerized Molecular Modeling for Discovering Promising Glycosaminoglycan Oligosaccharides that Modulate Protein Function

Imaging Glycosaminoglycan Modification Patterns In Vivo

Isolation and Purification of Versican and Analysis of Versican Proteolysis

Identification of Cell Autonomous and Non-Cell Autonomous Functions of Heparan Sulfate Glycosaminoglycan Chains by Creating Chimeric Mouse Embryos

Methods for Assessing the Effects of Xylosides on Angiogenesis

Role of HSPGs in Systemic Bacterial Infections

Generation of Drosophila Heparan Sulfate Mutant Cell Lines from Existing Fly Strains

Application of a Mutant Cell Library to Determine the Structure-Function Relationship of Heparan Sulfate in Facilitating FGF-FGFR Signaling

Manipulation of Glycosaminoglycans using Synthetic Xylosides to Study their Roles in Lung Branching Morphogenesis in Ex Vivo Lung Bud Culture System

Informatics Ecosystems to Advance the Biology of Glycans

Analysis of 3'-phosphoadenosine 5'-phosphosulfate Transporters: Transporter Activity Assay, Real-Time Reverse Transcription Polymerase Chain Reaction, and Immunohistochemistry

Preparation of Nanosensors for Detecting the Activity of Glycosaminoglycan Cleaving Enzymes

The Detection of Glycosaminoglycans in Pancreatic Islets and Lymphoid Tissues

Histochemical Analysis of Heparan Sulfate 3-O-sulfotransferase Expression in Mouse Brain

Mapping Proteoglycan Functions Using Novel Genetic Strategies

Xyloside Derivatives as Molecular Tools to Selectively Inhibit Heparan Sulfate and Chondroitin Sulfate Proteoglycan Biosynthesis

A Strategic Approach to Identification of Selective Inhibitors of Cancer Stem Cells

Applications of Xylosides in the Manipulation of Stem Cell Niche to Regulate Human Neural Stem Cell Differentiation and Neurite Outgrowth

Murine Models in the Evaluation of Heparan Sulfate-Based Anticoagulants

Investigating the Roles of Heparan Sulfate Structures in Alpha-Synuclein Aggregation in Cell Culture Models.

Other Format:

Printed edition:

ISBN:

978-1-0716-1398-6

9781071613986

Access Restriction:

Restricted for use by site license.