DNAzymes : Methods and Protocols / edited by Gerhard Steger, Hannah Rosenbach, Ingrid Span.

Format:

Book

Contributor:

Steger, Gerhard, Editor.

Rosenbach, Hannah, Editor.

Span, Ingrid, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2439

Methods in Molecular Biology, 1940-6029 ; 2439

Language:

English

Subjects (All):

Enzymology.

Genetics.

Genetics and Genomics.

Local Subjects:

Enzymology.

Genetics and Genomics.

Physical Description:

1 online resource (XVI, 312 pages) : 95 illustrations, 77 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume explores a collection of different protocols for the analysis and characterization of DNAzymes and their functions. The topics covered in this book range from bioinformatics and molecular dynamics simulations for the study or modification of nucleic acids to the descriptions of spectroscopic, fluorescence-based, or crystallographic methods to understand the structure and function of DNAzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, DNAzymes: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving field. .

Contents:

A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzyme

Selection of Aptamer for N-methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzyme

PCR Methods for the Generation of Catalytic ssDNA

Design of a DNAzyme: Prediction of mRNA Regions Accessible to a DNAzyme

Fluorescence-Based Kinetic Measurements for RNA-Cleaving DNAzymes

Stability and Activity of the 10-23 DNAzyme under Molecular Crowding Conditions

Quantifying the Number and Affinity of Mn2+-Binding Sites with EPR Spectroscopy

Obtaining Crystals of Nucleic Acids in Complex with the Protein U1A Using the Soaking Method

Crystallization and Structural Determination of 8-17 DNAzyme

Solution NMR Spectroscopy as a Tool to Study DNAzyme Structure and Function

Molecular Modeling and Simulations of DNA and RNA: DNAzyme as a Model System

Single-Molecule Kinetic Studies of Nucleic Acids by Förster Resonance Energy Transfer

Chemical Dual End-Labeling of Large Ribozymes

Spin Labeling of Long RNAs via Click Reaction and Enzymatic Ligation

Preparation of Site-Specifically Spin-Labeled RNA by In Vitro Transcription using an Expanded Genetic Alphabet

PELDOR Measurements on Nitroxide-Labelled Oligonucleotides

Sensing Metal Ions with Phosphorothioate-Modified DNAzymes

Enhancement of Activity for Peroxidase-Mimicking DNAzyme by Covalent Attachment of Hemin to G-quadruplex-Forming Oligonucleotide Using Click Chemistry

Generation of RNA with 2', 3'-cyclic Phosphates by Deoxyribozyme Cleavage in Frozen Solutions.

Other Format:

Printed edition:

ISBN:

978-1-0716-2047-2

9781071620472

Access Restriction:

Restricted for use by site license.