African Swine Fever Virus : Methods and Protocols / edited by Christopher L. Netherton.

Format:

Book

Contributor:

Netherton, Christopher L., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2503

Methods in Molecular Biology, 1940-6029 ; 2503

Language:

English

Subjects (All):

Virology.

Zoology.

Immunology.

Local Subjects:

Virology.

Zoology.

Immunology.

Physical Description:

1 online resource (XII, 218 pages) : 44 illustrations, 41 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field. .

Contents:

Working safely with African swine fever virus

Necropsy procedures and evaluation of macroscopic lesions of pigs infected with African swine fever virus

Preparation of immunofluorescently labelled tissue sections for imaging at low and high magnifications in the confocal microscope

Primary macrophage culture from porcine blood and lungs

Isolation of porcine bone marrow cells and generation of recombinant African swine fever viruses

Laboratory diagnosis and quantification of African swine fever virus using real-time polymerase chain reaction

Quantification of ASFV DNA and RNA in Ornithodoros soft ticks

Genotyping of African swine fever virus

African swine fever virus (ASFV) indirect ELISA test based on the use of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag)

Indirect immunoperoxidase test (IPT) for detection of antibodies against African swine fever virus (ASFV) on African green monkey cell lines (Vero, MS)

African Swine Fever Virus Haemadosrption Inhibition Assay

ELISpot assay for the detection of ASFV-specific interferon-gamma (IFN-γ) producing cells

Purification of African swine fever virus

African swine fever virus plaque assay and disinfectant testing

Bioorthogonal labelling of African swine fever virus infected cells

Whole genome sequencing of African swine fever virus.

Other Format:

Printed edition:

ISBN:

978-1-0716-2333-6

9781071623336

Access Restriction:

Restricted for use by site license.