Plant Proteases and Plant Cell Death : Methods and Protocols / edited by Marina Klemenčič, Simon Stael, Pitter F. Huesgen.

Format:

Book

Contributor:

Klemenčič, Marina, Editor.

Stael, Simon, Editor.

Huesgen, Pitter F., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2447

Methods in Molecular Biology, 1940-6029 ; 2447

Language:

English

Subjects (All):

Botany.

Plant Science.

Local Subjects:

Plant Science.

Physical Description:

1 online resource (XI, 299 pages) : 58 illustrations, 53 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume presents current methods to detect and measure the activity of proteolytic enzymes in organisms ranging from unicellular algae to flowering plants. Chapters detail in vitro production, characterization of plant proteases, tools for in vivo modifications, proteomic approaches for identification of substrates, inhibitors and interacting partners, and the function of plant proteases in plant programmed cell death. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Proteases: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. .

Contents:

Expression and purification of the type I metacaspase from a cryptophyte Guillardia theta, GtMCA-I

Expression and purification of the type II metacaspase from a unicellular green alga Chlamydomonas reinhardtii

Expression in Escherichia coli, refolding, and purification of plant aspartic proteases

Production of functional plant legumain proteases using the Leishmania tarentolae expression system

Purification of His-tagged proteases from the apoplast of agroinfiltrated N. benthamiana

Identification of cognate protease/substrate pairs by use of class-specific inhibitors

Peptide backbone modifications for the assessment of cleavage site relevance in precursors of signaling peptides

Detection of apoplastic protease inhibitors using convolution activity based proteome profiling

Cysteine reactivity profiling to unveil redox regulation in phytopathogens

Determination of caspase-like activities in roots by the use of fluorogenic substrates

Detection of damage-activated metacaspase activity by Western blot in plants

Sensitive plant N-terminome profiling with HUNTER

Profiling sequence specificity of proteolytic activities using proteome-derived peptide libraries

Monitoring Cell Death via Ion leakage and PAM Fluorometry

Measuring and perturbing ferroptosis in plants

Detection and quantification of the hypersensitive response cell death in Arabidopsis thaliana

Monitoring of ATG4 protease activity during autophagy in the model microalga Chlamydomonas reinhardtii

Oxidative stress in roots - detection of lipid peroxidation and total antioxidative capacity

Imaging of potassium and calcium distribution in plant tissues and cells to monitor stress response and programmed cell death

Monitoring Pseudomonas syringae growth in agroinfiltrated leaves: the 'agromonas' assay

Analysis of virus spread around the cell death zone at spatiotemporal resolution using confocal microscopy

A user guide to validation, annotation and evaluation of N-terminome datasets with MANTI

Exploring posttranslational modifications with the Plant PTM Viewer. .

Other Format:

Printed edition:

ISBN:

978-1-0716-2079-3

9781071620793

Access Restriction:

Restricted for use by site license.