Bioluminescence : Methods and Protocols, Volume 1 / edited by Sung-Bae Kim.

Format:

Book

Contributor:

Kim, Sung-Bae., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2524

Methods in Molecular Biology, 1940-6029 ; 2524

Language:

English

Subjects (All):

Biochemistry.

Materials-Analysis.

Imaging systems.

Imaging Techniques.

Local Subjects:

Biochemistry.

Imaging Techniques.

Physical Description:

1 online resource (XVI, 474 pages) : 130 illustrations, 108 illustrations in color.

Edition:

4th ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 1 delves into techniques for the establishment of luciferins and luciferases, basic in vitro and in vivo applications, as well as protocols on multiplex imaging platforms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 1 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.

Contents:

Gene Cloning and Functional Analysis of the Luciferase from Luminous Syllids of the Genus Odontosyllis

Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging

Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging

Biosynthesis-Inspired Deracemizative Production of D-Luciferin In Vitro by Combining Luciferase and Thioesterase

Production of Metridia Luciferase in Native Form by Oxidative Refolding from E. coli Inclusion Bodies

Production of Copepod Luciferases via Baculovirus Expression System

Molecular Tension Probe for In Vitro Bioassays

Optimized Loop-Mediated Amplification (LAMP) Allows Single Copy Detection Using Bioluminescent Assay in Real Time (BART)

A Simple and Rapid Bioluminescence-Based Functional Assay of Organic Anion Transporter 1 as a d-Luciferin Transporter

A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules against the Intracellular Form of Leishmania infantum

A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening against Infective African Trypanosomes

Imaging of Autonomous Bioluminescence Emission from Single Mammalian Cells

Rapid Single-Cell Detection of Beer-contaminating Lactic Acid Bacteria Using Bioluminescence/Rapid Microbe Detection

Bioluminescence of Aliivibrio fischeri in Artificial Seawater and Its Application in Fungicide Sensing

A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor

Design of an Intron-Retained Bioluminescence Reporter and Its Application in Imaging of Pre-mRNA Splicing in Living Subjects

Generation of Bi-Reporter Expressing Tri-Segmented Arenavirus

Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2

Generation, Characterization, and Applications of Influenza A Reporter Viruses

Optimized Aequorin Reconstitution Protocol to Visualize Calcium Ion Transients in the Heart of Transgenic Zebrafish Embryos In Vivo

Quantification and Imaging of Exosomes via Luciferase-Fused Exosome Marker Proteins: ExoLuc System

Bioluminescent Tracking of Human Induced Pluripotent Stem Cells In Vitro and In Vivo

Noninvasive In Vivo Tracking of Mammalian Cells Stably Expressing Firefly Luciferase

Bioluminescence Imaging for Evaluation of Antitumor Effect In Vitro and In Vivo in Mice Xenografted Tumor Models

Detection of Spontaneous Bone Metastases of Solid Human Tumor Xenografts in Mice

In Vivo Imaging Analysis of an Inner Ear Drug Delivery in Mice: Comparison of Inner Ear Drug Concentrations Over Time

Protocols for the Evaluation of a Lymphatic Drug Delivery System Combined with Bioluminescence to Treat Metastatic Lymph Nodes

In Vivo Bioluminescent Imaging of Rabies Virus Infection and Evaluation of Antiviral Drug

Imaging Infection by Vector-Borne Protozoan Parasites Using Whole-Mouse Bioluminescence

Longitudinal Tracing of Lyssavirus Infection in Mice via In Vivo Bioluminescence Imaging

Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Anti-Relapse Compounds

Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase Reporter Plasmids

Multiplex Hextuple Luciferase Assaying

Molecular Imaging of Tumor Progression and Angiogenesis by Dual Bioluminescence.

Other Format:

Printed edition:

ISBN:

978-1-0716-2453-1

9781071624531

Access Restriction:

Restricted for use by site license.