Plant Pathology : Method and Protocols / edited by Nicola Luchi.

Format:

Book

Contributor:

Luchi, Nicola, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2536

Methods in Molecular Biology, 1940-6029 ; 2536

Language:

English

Subjects (All):

Botany.

Plant diseases.

Plant Science.

Plant Pathology.

Local Subjects:

Plant Science.

Plant Pathology.

Physical Description:

1 online resource (XVIII, 497 pages) : 106 illustrations, 96 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume covers the latest developments in different areas of plant pathology. The chapters in this volume are organized into seven parts. Part One provides traditional methods for isolation and identification of invasive pathogens and root disease. Part Two looks at new and rapid DNA extraction protocols from different samples, and Part Three focuses on molecular detection protocols for identifying and quantifying plant pathogens, including fungal and bacterial invasive species. Part Four describes the application of metabarcoding in plant pathology, and Part Five talks about plant pathogen interactions. Part Six concentrates on population genomics of plant pathogens, and Part Seven covers biocontrol on plant pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plant Pathology: Methods and Protocols is a valuable resource for researchers in the plant pathology community, and discusses various approaches for the detection, identification, and control of plant diseases.

Contents:

Obtaining and Maintaining Cultures of Pinewood Nematodes Bursaphelenchus xylophilus from Wild Dauers

Survey and Monitoring of Phytophthora Species in Natural Ecosystems: Methods for Sampling, Isolation, Purification, Storage, and Pathogenicity Tests

Field and Laboratory Procedures for Fusarium circinatum Identification and Diagnosis

Rapid Extraction of Plant Nucleic Acids by Microneedle Patch for In-Field Detection of Plant Pathogens

DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues

A Rapid Method for Extracting High Quality DNA from Soils

Invasive Alien Plant Pathogens: The Need of New Detection Methods

Detection of Airborne Inoculum of Hymenoscyphus fraxineus: The Causal Agent of Ash Dieback

Molecular Detection of Wheat Blast Pathogen in Seeds

Detection and Identification of the Causal Agents of Dothistroma Needle Blight

The Use of qPCR to Detect Cryphonectria parasitica in Plants

Rapid Molecular Diagnostics in the Field and Laboratory to Detect Plant Pathogen DNA in Potential Insect Vectors

A Panel of Real-Time PCR Assays for the Direct Detection of All the Xylella fastidiosa Subspecies

Selective Quantification of Erwinia amylovora Live Cells in Pome Fruit Tree Cankers by Viability Digital PCR

Innovative Detection of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens, Causal Agent of Bacterial Wilt of Leguminous Plants

Specific Detection of Pseudomonas savastanoi pathovars: From End-Point PCR to Real-Time PCR and HRMA

Computational Analysis of HTS Data and Its Application in Plant Pathology

Biomonitoring of Fungal and Oomycete Plant Pathogens by Using Metabarcoding

Phytobiome Metabarcoding: A Tool to Help Identify Prokaryotic and Eukaryotic Causal Agents of Undiagnosed Tree Diseases

Plant-Fungal Interactions: Laser Microdissection as a Tool to Study Cell Specificity

Somatic Embryogenesis as a Tool for Studying Grapevine-Virus Interaction

A Quantitative Luminol-Based Assay for ROS Burst Detection in Potato Leaves in Response to Biotic Stimuli

Genetic Analysis of Plant Pathogens Natural Populations

Microsatellite Genotyping in the Chestnut Blight Fungus Cryphonectria parasitica

A Multiplex PCR Approach to Determine Vegetative Incompatibility Genotypes and Mating Type in Cryphonectria parasitica

A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents

Spray-Induced Gene Silencing to Study Gene Function in Phytophthora

Plant Sampling for Production of Essential Oil and Evaluation of Its Antimicrobial Activity In Vitro.

Other Format:

Printed edition:

ISBN:

978-1-0716-2517-0

9781071625170

Access Restriction:

Restricted for use by site license.