Rhodopsin : Methods and Protocols / edited by Valentin Gordeliy.

Format:

Book

Contributor:

Gordeliy, Valentin, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2501

Methods in Molecular Biology, 1940-6029 ; 2501

Language:

English

Subjects (All):

Microbiology.

Local Subjects:

Microbiology.

Physical Description:

1 online resource (XI, 363 pages) : 66 illustrations, 64 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume provides readers with the latest information on the advances made in the field of rhodopsins. The chapters in this book cover topics such as new discoveries and developments; new ways to search for rhodopsins; methods to characterize the function and structure of rhodopsins on a molecular level; nano volume high throughput in meso crystallization, and fourth generation x-ray synchrotron sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Rhodopsin: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.

Contents:

Microbial Rhodopsins

Molecular Biology of Microbial Rhodopsins

Rhodopsin-Based Optogenetics: Basics and Applications

Searching Metagenomes for New Rhodopsins

E. coli Expression and Purification of Microbial and Viral Rhodopsins

Crystallization of Microbial Rhodopsins

Crystallographic Studies of Rhodopsins: Structure and Dynamics

Time-Resolved UV-VIS Spectroscopy of Microbial Rhodopsins

Solid-State NMR Spectroscopy on Microbial Rhodopsins

FTIR and Raman Spectroscopy of Rhodopsins

Single Cell Resolution Optogenetics via Expression of Soma-Targeted Rhodopsins

Electrophysiological Characterization of Microbial Rhodopsins Transport Properties: Electrometric and ΔpH Measurements using Planar Lipid Bilayer, Collodion Film and Fluorescent Probes Approaches

Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments

Molecular Optimization of Rhodopsin-Based Tools for Neuroscience Applications

Optogenetic Studies of Mitochondria

In Vivo and In Vitro Characterization of Cyclase and Phosphodiesterase Rhodopsins

Optogenetic Control of Human Stem Cell-Derived Neurons.

Other Format:

Printed edition:

ISBN:

978-1-0716-2329-9

9781071623299

Access Restriction:

Restricted for use by site license.