Embryonic Stem Cell Protocols / edited by Kursad Turksen.

Format:

Book

Contributor:

Turksen, Kursad, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2520

Methods in Molecular Biology, 1940-6029 ; 2520

Language:

English

Subjects (All):

Developmental biology.

Stem cells.

Cytology.

Developmental Biology and Stem Cells.

Stem Cell Biology.

Cell Biology.

Local Subjects:

Developmental Biology and Stem Cells.

Stem Cell Biology.

Cell Biology.

Physical Description:

1 online resource (XIII, 363 pages) : 1 illustrations

Edition:

4th ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Embryonic Stem Cell Protocols, Fourth Edition serves as an ideal resource for researching mining the depths of the ESC field.

Contents:

Dissecting Molecular Phenotypes through FACS-Based Pooled CRISPR Screens

Feeder Free Human Embryonic Stem Cell Culture under Defined Culture Conditions

Rat Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish

Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells

High-Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model

Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications

Feeder Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol

ChIP-qPCR for Polycomb Group Proteins during Neuronal Differentiation of Human Pluripotent Stem Cells

Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids

Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs)

Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo

Human Trophectoderm Spheroid Derived from Human Embryonic Stem Cells

Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions

A Simple, Rapid, and Cost-effective Method for Loss-of-Function Assays in Pluripotent Cells

Embryoid Body Formation from Mouse and Human Pluripotent Stem Cells for Transplantation to Study Brain Microenvironment and Cellular Differentiation

A Modified SMART-Seq Method for Single Cell Transcriptomic Analysis of Embryoid Body Differentiation

Osteogenic Differentiation from Mouse Embryonic Stem Cells

Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation

Mouse Embryonic Stem Cell Culture in Serum-Containing or 2i Conditions

Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages

Accessing the Human Pluripotent Stem Cell Translatome by Polysome Profiling

Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors

Proteomic Analysis of Human Neural Stem Cells Differentiation by SWATH-MS.

Other Format:

Printed edition:

ISBN:

978-1-0716-2437-1

9781071624371

Access Restriction:

Restricted for use by site license.