Malaria Immunology : Targeting the Surface of Infected Erythrocytes / edited by Anja Tatiana Ramstedt Jensen, Lars Hviid.

Format:

Book

Contributor:

Jensen, Anja Tatiana Ramstedt, Editor.

Hviid, Lars, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2470

Methods in Molecular Biology, 1940-6029 ; 2470

Language:

English

Subjects (All):

Immunology.

Virology.

Local Subjects:

Immunology.

Virology.

Physical Description:

1 online resource (XIX, 796 pages) : 144 illustrations, 124 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume covers a broad range of methods, technologies, and protocols on malaria. Chapters detail research on collecting parasites in the field, single molecule-level analyses of adhesive interactions, and focused studies aiming at disrupting the devastating disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Malaria Immunology: Targeting the Surface of Infected Erythrocytes aims to be a useful and practical guide to researches to help further their study in this field. Chapter Analysis of var gene transcription pattern using DBLα-tags [Chapter 14] is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Identification and enumeration of Plasmodium falciparum parasites by light microscopy

Collection and cryopreservation of Plasmodium falciparum clinical isolates in the field

Preservation of parasite RNA in the field

Preservation and extraction of malaria parasite DNA from dried blood spots

Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum

Genotyping of Plasmodium falciparum isolates to assess clone composition in parasite cultures

Immunomagnetic selection of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants

Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants

Selecting Plasmodium falciparum infected erythrocytes for adhesion to cell lines

Synchronisation of Plasmodium falciparum and P. knowlesi in vitro cultures using a highly specific protein kinase inhibitor

Generation of Plasmodium falciparum gametocytes in vitro with specific considerations for field isolates

Imaging of extracellular vesicles derived from Plasmodium falciparum-infected red blood cells using atomic force microscopy

Analysis of var gene transcript patterns by quantitative real-time PCR

Analysis of var gene transcription pattern using DBLα-tags

RNAseq of infected erythrocyte surface antigen-encoding genes

Resetting var gene transcription in Plasmodium falciparum

CRISPR/Cas9 editing of the Plasmodium falciparum genome

CRISPR-Cas9 editing of the Plasmodium falciparum genome - Special applications

Extraction and immunoprecipitation of VAR2CSA, the PfEMP1 associated with placental malaria

Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system

Expression of large full-length PfEMP1 proteins in HEK293 cells

Receptor affinity-based purification of PfEMP1 proteins

Analysis of antibody reactivity to malaria antigens by microsphere-based multiplex immunoassay

High-throughput BioPlex assay for the study of functionally active Plasmodium falciparum antigens that are expressed on the surface of infected erythrocytes

Protein microarrays as a tool to analyze antibody responses to variant surface antigens expressed on the surface of Plasmodium falciparum-infected erythrocytes

Protocol for differential biopanning of Plasmodium falciparum phage display cDNA library to identify parasite targets of protective antibodies

Affinity purification of PfEMP1-specific antibodies from human blood

Production of anti-PfEMP1 polyclonal antisera in rats and mice

Production of PfEMP1-specific mouse monoclonal antibodie

Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals

Visualization of infected red blood cell surface antigens by fluorescence microscop

Analysis by flow cytometry of α2‑macroglobulin and non-immune IgM-binding to Plasmodium falciparum-infected erythrocytes

An improved method for assessing antigen presentation on the surface of Plasmodium falciparum-infected erythrocytes by immuno-electron microscopy

Assessing antigen presentation on the surface of Plasmodium falciparum-infected erythrocytes by photoactivated localization microscopy (PALM)

Surface plasmon resonance analysis of PfEMP1 interaction with receptors

Analysis of antibody neutralization of PfEMP1 binding by competition ELISA

Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay

Bridging and clumping: investigating platelet interactions with Plasmodium falciparum-infected red blood cells and endothelial cells in cerebral malaria

Assay of static adhesion of Plasmodium falciparum-infected erythrocytes to cells, including inhibition of the adhesion

Static adhesion of Plasmodium falciparum-infected erythrocytes to purified and recombinant receptors

Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions

Chip-based assay of adhesion of Plasmodium falciparum-infected erythrocytes to cells under flow

Binding of Plasmodium falciparum-infected red blood cells to engineered 3D microvessels

3D organoid assay of the impact of Plasmodium falciparum-infected erythrocyte adhesion on the blood-brain barrier

Receptor-functionalized lipid membranes as biomimetic surfaces for adhesion of Plasmodium falciparum-infected erythrocytes

Antibody-dependent THP-1 cell-mediated phagocytosis of Plasmodium falciparum-infected erythrocytes

Assaying interactions between neutrophils and Plasmodium falciparum-infected red blood cells

Natural killer cell antibody dependent cellular cytotoxicity (ADCC) activity against Plasmodium falciparum-infected red blood cells

Assessing PfGARP-mediated apoptosis of blood-stage Plasmodium falciparum parasites

Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies

Flow cytometry assay of Plasmodium falciparum-specific B-cell proportions

Assessment of Plasmodium falciparum antigen-specific B cells

Whole blood dendritic cell cytokine production assay

Measuring the impact of malaria on the living human retina

Ocular pathology of cerebral malaria

Investigating interactions between endothelial cells and parasitised red blood cells in skin and subcutaneous tissue. .

Other Format:

Printed edition:

ISBN:

978-1-0716-2189-9

9781071621899

Access Restriction:

Restricted for use by site license.