Alternative Splicing : Methods and Protocols / edited by Peter Scheiffele, Oriane Mauger.

Format:

Book

Contributor:

Scheiffele, Peter, Editor.

Mauger, Oriane, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2537

Methods in Molecular Biology, 1940-6029 ; 2537

Language:

English

Subjects (All):

Genetics.

Genomics.

Genetics and Genomics.

Local Subjects:

Genetics and Genomics.

Genomics.

Physical Description:

1 online resource (XI, 354 pages) : 61 illustrations, 57 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.

Contents:

Alternative Splicing in Human Biology and Disease

Targeting Alternative Splicing for Therapeutic Interventions

An Optimized Protocol for the Mapping of Cell Type-Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions

FACS-Based Neuronal Cell Type-Specific RNA Isolation and Alternative Splicing Analysis

Quantitative Measurement of Alternatively Spliced RNA Isoform Levels

Analysis of Splicing Regulation by Third-Generation Sequencing

Computational Analysis of Alternative Splicing Using VAST-TOOLS and the VastDB Framework

Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data

Functional Annotation of Custom Transcriptomes

Design, Labeling, and Application of Probes for RNA smFISH

BaseScope™ Approach to Visualize Alternative Splice Variants in Tissue

In Situ Imaging of mRNA Splicing Variants by SpliceRCA

Two-Color Fluorescent Reporters for Analysis of Alternative Splicing

Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectrometry

Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscopy

Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention

Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins

Probing Liquid-Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo

Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals.

Other Format:

Printed edition:

ISBN:

978-1-0716-2521-7

9781071625217

Access Restriction:

Restricted for use by site license.