Prokaryotic Gene Regulation : Methods and Protocols / edited by Eveline Peeters, Indra Bervoets.

Format:

Book

Contributor:

Peeters, Eveline, Editor.

Bervoets, Indra, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2516

Methods in Molecular Biology, 1940-6029 ; 2516

Language:

English

Subjects (All):

Microbial genetics.

Bioinformatics.

Microbial Genetics.

Computational and Systems Biology.

Local Subjects:

Microbial Genetics.

Computational and Systems Biology.

Physical Description:

1 online resource (X, 318 pages) : 56 illustrations, 44 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal chromosome, the main template for all gene regulatory processes, and its epigenetic modification. Section Two looks at a selection of approaches that enable higher levels of understanding of transcription initiation, a key step in information processing. Section Three discusses the investigation of regulating transcription factors, which are often considered the main players in gene regulation in prokaryotic cells. The Fourth Section focuses on the next stage of information processing at which gene regulation occurs, namely the RNA-based level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Prokaryotic Gene Regulation: Methods and Protocols is a valuable resource for researchers interested in learning more about this diverse field.

Contents:

Chromosome Conformation Capture in Bacteria and Archaea

Micrococcal Nuclease Digestion Assays for the Analysis of Chromosome Structure in Archaea

Detecting DNA Methylations in the Hyperthermoacidophilic Crenarchaeon Sulfolobus acidocaldarius using SMRT-Sequencing

ProD: A Tool for Predictive Design of Tailored Promoters in Escherichia coli

Computational Study on the Dynamics of Mycobacterium Tuberculosis RNA Polymerase Assembly

In Vitro Transcription Assay for Archaea Belonging to Sulfolobales

Prediction of DNA-Binding Transcription Factors in Bacteria and Archaea Genomes

In Vivo Screening Method for the Identification and Characterization of Prokaryotic, Metabolite-Responsive Transcription Factors

Application of Special Electron Microscopy Techniques to the Study of DNA-Protein Complexes in E. coli Cells

High-Speed Atomic Force Microscopy Visualization of Protein-DNA Interactions using DNA Origami Frames

Separation and Characterization of Protein-DNA Complexes by EMSA and In-Gel Footprinting

Chemical Protection and Premodification Binding Interference for the Identification of Phosphate and Base-Specific Contacts in Protein-DNA Complexes

Analysis of Protein-DNA Interactions using Isothermal Titration Calorimetry: Successes and Failures

Determination of RNA Structure with In Vitro SHAPE-Experiments

Microscale Thermophoresis to Study RNA-RNA Binding Affinity

Toeprint Assays for Detecting RNA Structure and Protein-RNA Interactions.

Other Format:

Printed edition:

ISBN:

978-1-0716-2413-5

9781071624135

Access Restriction:

Restricted for use by site license.