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Single Cell 'Omics of Neuronal Cells / edited by Jonathan V. Sweedler, James Eberwine, Scott E. Fraser.
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Neuromethods 1940-6045 ; 184
- Neuromethods, 1940-6045 ; 184
- Language:
- English
- Subjects (All):
- Neurosciences.
- Neurophysiology.
- Neuroscience.
- Local Subjects:
- Neuroscience.
- Neurophysiology.
- Physical Description:
- 1 online resource (XIV, 294 pages) : 58 illustrations, 56 illustrations in color.
- Edition:
- 1st ed. 2022.
- Contained In:
- Springer Nature eBook
- Place of Publication:
- New York, NY : Springer US : Imprint: Humana, 2022.
- System Details:
- text file PDF
- Summary:
- This volume discusses the latest techniques used in the diverse fields of single cell 'omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory. Cutting-edge and comprehensive, Single Cell 'Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.
- Contents:
- Singe Cell aRNA-Seq for Transcriptomics and Cellular Phenotyping
- Single Cell Genomic and Epigenomic DNA Analyses
- Single-Cell Proteome Profiling of Neuronal Cells
- Joint Analysis of Transcriptome and Proteome Measurements in Single Cells with TotalVI
- Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional Biology
- High-Throughout Image-Guided Microprobe Mass Spectrometric Analysis of Single Cells
- Single-Cell Resolution Immunoblotting
- Quantifying Enzyme Activity and Gene Expression within Single-Cells using a Multiplexed Capillary Electrophoresis Platform
- Quantitative Chemical Imaging at the Cell Level-SIMS, Fluorescence, and Correlative Techniques
- Electrophysiology and Single Cells
- Genetically Encoded Biosensors Reveal Spatiotemporal Dynamics and Cellular Heterogeneity of Neuronal Cells.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-0716-2525-5
- 9781071625255
- Access Restriction:
- Restricted for use by site license.
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