Bacterial Amyloids : Methods and Protocols / edited by Véronique Arluison, Frank Wien, Andrés Marcoleta.

Format:

Book

Contributor:

Arluison, Véronique, Editor.

Wien, Frank, Editor.

Marcoleta, Andrés, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2538

Methods in Molecular Biology, 1940-6029 ; 2538

Language:

English

Subjects (All):

Proteins.

Bacteria.

Local Subjects:

Proteins.

Bacteria.

Physical Description:

1 online resource (XI, 337 pages) : 108 illustrations, 98 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This detailed volume introduces different methods to assess the structure and folding of bacterial amyloids and analyze their functions, either in vitro or in vivo. Despite their initial association with neurodegenerative diseases, there is now increasing evidence of alternative roles for amyloids, with beneficial aspects for cells. In particular, prokaryotes use amyloids as functional assemblies, where they are key players in the cell's physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Bacterial Amyloids: Methods and Protocols is an ideal guide for experts and novices alike to further explore these crucial protein assemblies.

Contents:

Study of Amyloid Fibers Using Atomic Force Microscopy

Cryo-Electron Microscopy to Analyze the Structure of Bacterial Amyloids In Vitro

Monitoring Molecular Assembly of Biofilms Using Quartz Crystal Microbalance with Dissipation (QCM-D)

Measuring the Size and Spontaneous Fluctuations of Amyloid Aggregates with Fluorescence Correlation Spectroscopy

Determining the Stoichiometry of Amyloid Oligomers by Single Molecule Photobleaching

Structural Information on Bacterial Amyloid and Amyloid-DNA Complex Obtained by Small-Angle Neutron/X-Ray Scattering

Fiber Diffraction and Small-Angle Scattering for Structural Investigation of Bacterial Amyloids

Supported Lipid Bilayers (SLBs) to Study Amyloid-Lipid Membrane Interactions with Atomic Force Microscopy

Characterization of Bacterial Amyloids by Nano-Infrared Spectroscopy

High Throughput Screening of Heterologous Functional Amyloids Using Escherichia coli

Evaluation of Amyloid Inhibitor Efficiency to Block Bacterial Survival

Anti-Amyloid Drug Screening Methods Using Bacterial Inclusion Bodies

Determination of Effects and Mechanisms of Action of Bacterial Amyloids on Antibiotic Resistance

Design and Testing of Synthetic Catalytic Amyloids Based on the Active Site of Enzymes

Analysis of Bacterial Amyloid Interaction with Lipidic Membrane by Orientated Circular Dichroism and Infrared Spectroscopies

Direct, Rapid, and Simple Evaluation of the Expression and Conformation of Beta-Amyloid in Bacterial Cells by FTIR Spectroscopy

Assessment of Intracellular Amyloid Formation in Fixed and Live Bacteria Using Fluorescence Microscopy

Identification of Aggregation-Prone and Gatekeeper Residues in Bacterial Amyloids Using Site-Directed Mutagenesis and Flow Cytometry

Probing DNA-Amyloid Interaction and Gel Formation by Active Magnetic Wire Microrheology

Probing Amyloids-DNA Interaction with Nanofluidics

Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo Soft X-Ray Tomography.

Other Format:

Printed edition:

ISBN:

978-1-0716-2529-3

9781071625293

Access Restriction:

Restricted for use by site license.