Lipid Rafts : Methods and Protocols / edited by Erhard Bieberich.

Format:

Book

Contributor:

Bieberich, Erhard, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2187

Methods in Molecular Biology, 1940-6029 ; 2187

Language:

English

Subjects (All):

Lipids.

Mass spectrometry.

Lipidology.

Mass Spectrometry.

Local Subjects:

Lipidology.

Mass Spectrometry.

Physical Description:

1 online resource (XI, 350 pages) : 79 illustrations, 62 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning more about the function of lipid rafts in many areas of cell biology and medicine. .

Contents:

Isolation and Analysis of Lipid Rafts from Neural Cells and Tissues

A Detergent-Free Method for Preparation of Lipid Rafts for the Shotgun Lipidomics Study

Biochemical Analysis of Lipid Rafts to Study Pathogenic Mechanisms of Neural Diseases

Amyloid- β Interactions with Lipid Rafts in Biomimetic Systems: A Review of Laboratory Methods

Extracellular Vesicles Containing Ceramide-Rich Platforms: "Mobile Raft" Isolation and Analysis

Isolation of Lipid Rafts (Detergent-Resistant Microdomains) and Comparison to Extracellular Vesicles (Exosomes)

Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics

Association of Glycolipids and Growth Factor Receptors in Lipid Rafts

Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts during Apoptosis Triggering

Elucidating the Role of Lipid Rafts on G Protein-Coupled Receptor Function in the Mouse Kidney: An In Vivo Approach

Analysis of Lipids in Ceramide-Enriched Membrane Domains

Chemotherapeutic Agents-Induced Ceramide Rich Platforms (CRPs) in Endothelial Cells and their Modulation

Biophysical Analysis of Lipid Domains by Fluorescence Microscopy

Biophysical Analysis of Lipid Domains in Mammalian and Yeast Membranes by Fluorescence Spectroscopy

Characterization of Lipid Order and Domain Formation in Model Membranes Using Fluorescence Microscopy and Spectroscopy

Characterization of the Effect of Sphingolipid Accumulation on Membrane Compactness, Dipole Potential, and Mobility of Membrane Components

Super-Resolution Microscopy of Sphingolipids

Detection of Functionalized Sphingolipid Analogues in Detergent Resistant Membranes of the Immune Cells

Immunofluorescence Labeling of Lipid-Binding Proteins CERTs to Monitor Lipid Rafts Dynamics

Cross-Link/Proximity Ligation Assay for Visualization of Lipid and Protein Complexes in Lipid Rafts.

Other Format:

Printed edition:

ISBN:

978-1-0716-0814-2

9781071608142

Access Restriction:

Restricted for use by site license.