Periodontal Pathogens : Methods and Protocols / edited by Keiji Nagano, Yoshiaki Hasegawa.

Format:

Book

Contributor:

Nagano, Keiji, Editor.

Hasegawa, Yoshiaki, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2210

Methods in Molecular Biology, 1940-6029 ; 2210

Language:

English

Subjects (All):

Microbiology.

Dentistry.

Medicine-Research.

Biology-Research.

Biomedical Research.

Local Subjects:

Microbiology.

Dentistry.

Biomedical Research.

Physical Description:

1 online resource (XI, 256 pages) : 58 illustrations, 31 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This book addresses the major periodontal pathogens implicated as causal agents in periodontal disease, including Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Prevotella spp. Beginning with methods for bacterial genetic manipulation, the volume continues with sections on experimental methods to examine virulence factors, interactions with other pathogenic microorganism and host cells, as well as a chapter on an animal model of periodontitis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Periodontal Pathogens: Methods and Protocols serves as an extensive and useful reference for researchers studying periodontal pathogens and will help elucidate the causes of periodontal disease and the systemic diseases related to it.

Contents:

Site-Directed and Random Mutagenesis in Porphyromonas gingivalis: Construction of Fimbriae-Related-Gene Mutant

Genetic Manipulations of Oral Spirochete Treponema denticola

Construction of a Gene-Deletion Mutant in Tannerella forsythia

Construction of a Mutant in Prevotella melaninogenica Using the Conjugation Transfer Method with Escherichia coli

Genetic Transformation of Fusobacterium nucleatum

Genotyping of Porphyromonas gingivalis in Relationship to Virulence

Transport and Polymerization of Porphyromonas gingivalis Type V Pili

Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis

Crystallization of Recombinant Fimbrial Proteins of Porphyromonas gingivalis

Enzymatic Characteristics and Activities of Gingipains from Porphyromonas gingivalis

Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis

Methods for Functional Characterization of the Type IX Secretion System of Porphyromonas gingivalis

Purification of Tannerella forsythia Surface (S-) Layer Proteins

Separation of Glycosylated OmpA-Like Proteins from Porphyromonas gingivalis and Tannerella forsythia

Intranasal Vaccine Study Using Porphyromonas gingivalis Membrane Vesicles: Isolation Method and Application to a Mouse Model

Analysis of the Butyrate-Producing Pathway in Porphyromonas gingivalis

Characterization of the Treponema denticola Virulence Factor Dentilisin

Evaluation of the Virulence of Aggregatibacter actinomycetemcomitans through the Analysis of Leukotoxin

Lipoprotein Extraction from Microbial Membrane and Lipoprotein/Lipopeptide Transfection into Mammalian Cells

Analysis of the Interaction between HIV and Periodontopathic Bacteria that Reactivates HIV Replication in Latently Infected Cells

Invasion of Gingival Epithelial Cells by Porphyromonas gingivalis

Analysis of Interaction between Porphyromonas gingivalis and Endothelial Cells In Vitro

Analysis of Experimental Ligature-Induced Periodontitis Model in Mice.

Other Format:

Printed edition:

ISBN:

978-1-0716-0939-2

9781071609392

Access Restriction:

Restricted for use by site license.