Capturing Chromosome Conformation : Methods and Protocols / edited by Beatrice Bodega, Chiara Lanzuolo.

Format:

Book

Contributor:

Bodega, Beatrice., Editor.

Lanzuolo, Chiara., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2157

Methods in Molecular Biology, 1940-6029 ; 2157

Language:

English

Subjects (All):

Biology-Technique.

Cytology.

Genetics.

Biological Techniques.

Cell Biology.

Genetics and Genomics.

Local Subjects:

Biological Techniques.

Cell Biology.

Genetics and Genomics.

Physical Description:

1 online resource (XI, 322 pages) : 66 illustrations, 54 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization.

Contents:

Capturing Chromosome Conformation

The Chromosome Conformation Capture (3C) in Drosophila melanogaster

4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture

Analysis, Modeling, and Visualization of Chromosome Conformation Capture Experiments

Targeted DNase Hi-C

C-HiC: A High Resolution Method for Unbiased Chromatin Conformation Capture Targeting Small Locus

Computational Analysis of Hi-C Data

Profiling Chromatin Landscape at High Resolution and Throughput with 2C-ChIP

Single-Cell DamID to Capture Contacts between DNA and the Nuclear Lamina in Individual Mammalian Cells

Formaldehyde-Mediated Snapshot of Nuclear Architecture

Visualization of Chromatin Dynamics by Live Cell Microscopy Using CRISPR/Cas9 Gene Editing and ANCHOR Labeling

Preparing Map of Chromosome Territories Distribution Frequency

Higher-Order Chromatin Organization Using 3D DNA Fluorescent In Situ Hybridization

3D Immuno-DNA Fluorescence In Situ Hybridization (FISH) for Detection of HIV-1 and Cellular Genes in Primary CD4+ T Cells

Visualization of Nuclear and Cytoplasmic Long Noncoding RNAs at Single-Cell Level by RNA-FISH

3D COMBO chrRNA-DNA-Immuno FISH

An Algorithm for the Analysis of the 3D Spatial Organization of the Genome.

Other Format:

Printed edition:

ISBN:

978-1-0716-0664-3

9781071606643

Access Restriction:

Restricted for use by site license.