Cardiac Regeneration : Methods and Protocols / edited by Kenneth D. Poss, Bernhard Kühn.

Format:

Book

Contributor:

Poss, Kenneth D., Editor.

Kühn, Bernhard., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2158

Methods in Molecular Biology, 1940-6029 ; 2158

Language:

English

Subjects (All):

Regenerative medicine.

Physiology.

Biology-Technique.

Regenerative Medicine and Tissue Engineering.

Biological Techniques.

Local Subjects:

Regenerative Medicine and Tissue Engineering.

Physiology.

Biological Techniques.

Physical Description:

1 online resource (XIII, 346 pages) : 83 illustrations, 69 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them. The chapter "Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Myocardial Infarction Techniques in Adult Mice

Apical Resection and Cryoinjury in Neonatal Mouse Heart

Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice

Myocardial Infarction in Pigs

Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish

Cardiac Resection Injury in Zebrafish

A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish

Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration

Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes

Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes

Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells

In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells

Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs

Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells

Isolation and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts

Ex Vivo Techniques to Study Heart Regeneration in Zebrafish

Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters

In Vivo Clonal Analysis of Cardiomyocytes

High Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry

AAV Gene Transfer to the Heart

In Vitro Synthesis of Modified RNA for Cardiac Gene Therapy

Generation and Manipulation of Exosomes

Epigenetic Assays in Purified Cardiomyocyte Nuclei

Genetic Lineage Tracing of Non-Cardiomyocytes in Mice

Experimental Hypoxia as a Model for Cardiac Regeneration in Mice.

Other Format:

Printed edition:

ISBN:

978-1-0716-0668-1

9781071606681

Access Restriction:

Restricted for use by site license.