Reactive Oxygen Species : Methods and Protocols / edited by Jesús Espada.

Format:

Book

Contributor:

Espada, Jesús, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2202

Methods in Molecular Biology, 1940-6029 ; 2202

Language:

English

Subjects (All):

Biochemistry.

Local Subjects:

Biochemistry.

Physical Description:

1 online resource (XI, 233 pages) : 63 illustrations, 42 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

Introduction to in silico Modeling to Study ROS Dynamics

Assessment of ROS Production in Mitochondria of Live Cells

Measurement of Superoxide Production in Acute Hypoxia by Fixed-Cell Microscopy

A Photodynamic Tool to Promote a Sustained, ROS-Dependent Growth of Human Hair Follicles in ex vivo Culture

Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana

Characterization of oxidative lipidomics and autophagy induction in Chlamydomonas reinhardtii under abiotic stress

Fluorescence Detection of Increased Reactive Oxygen Species Levels in Saccharomyces cerevisiae at the Diauxic Shift

Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa

Measurement of Reactive Oxygen Species In Semen Samples Using Chemiluminescence

Methods to Unravel Pathways of Reactive Oxygen Species in the Photodynamic Inactivation of Bacteria

Revealing ROS Production by Antibiotics and Photosensitizers in Biofilms. A Fluorescence Microscopy Approach

EPR Spectroscopy as a Method for ROS Quantification in Skin

Detection of Superoxide Radical in Adherent Living Cells by Electron Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones

Singlet Oxygen Quantum Yield Determination Using Chemical Acceptors

A General Method to Establish the Relative Efficiency of Different Sonosensitizers to Generate ROS for SDT

Lipid Peroxidation Assay using BODIPY-phenylbutadiene Probes:A Methodological Overview

Use of Fluorescent Probes to Detect ROS in Photodynamic Therapy. .

Other Format:

Printed edition:

ISBN:

978-1-0716-0896-8

9781071608968

Access Restriction:

Restricted for use by site license.