Channelrhodopsin : Methods and Protocols / edited by Robert E. Dempski.

Format:

Book

Contributor:

Dempski, Robert E., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2191

Methods in Molecular Biology, 1940-6029 ; 2191

Language:

English

Subjects (All):

Proteins.

Microbiology.

Protein Biochemistry.

Local Subjects:

Protein Biochemistry.

Microbiology.

Physical Description:

1 online resource (XII, 405 pages) : 117 illustrations, 99 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This book merges approaches in understanding the function of the light-gated ion channels known as channelrhodopsin together with methods addressing how channelrhodopsins can be used to address biomedical questions on a cellular or organismal level. Since the first molecular identification of channelrhodopsins, a broad range of tools have been created and new approaches developed to both better understand the molecular determinants of channelrhodopsin function as well as to use these and homologous proteins from a variety of species as tools to better understand physiological processes, which this volume addresses. Additionally, channelrhodopsins have become instrumental as a potential treatment for disease states. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Channelrhodopsin: Methods and Protocols provides a resource for those interested in honing their current expertise in this vital area of study as well as potentially branching out into new directions.

Contents:

Molecular Dynamics Simulations of Channelrhodopsin Chimera, C1C2

Computing Potential of the Mean Force Profiles for Ion Permeation through Channelrhodopsin Chimera, C1C2

Nanodisc Reconstitution of Channelrhodopsins Heterologously Expressed in Pichia pastoris for Biophysical Investigations

Characterizing Channelrhodopsin Channel Properties via Two-Electrode Voltage Clamp and Kinetic Modeling

Charge Transport by Light-Activated Rhodopsins Determined by Electrophysiological Recordings

Probing Channelrhodopsin Electrical Activity in Algal Cell Populations

Two-Photon Optogenetic Stimulation of Drosophila Neurons

Probing Synaptic Signaling with Optogenetic Stimulation and Genetically-Encoded Calcium Reporters

Optogenetics to Interrogate Neuron-Glia Interactions in Pups and Adults

Chronic Optogenetic Pacing of Human Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues

Patterned Optogenetic Stimulation Using a DMD-Projector

Selecting Channelrhodopsin Constructs for Optimal Visual Restoration in Differing Light Conditions

Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish

Automated Functional Screening for Modulators of Optogenetically-Activated Neural Responses in Living Organisms

Optogenetic Interrogation of ChR2-Expressing GABAergic Interneurons after Transplantation into the Mouse Brain

Application of Targeting-Optimized Chronos for Stimulation of the Auditory Pathway

Channelrhodopsins for Cell-Type Specific Illumination of Cardiac Electrophysiology

Optogenetic Control of Cardiac Autonomic Neurons in Transgenic Mice

Dissecting Mechanisms of Motivation within the Nucleus Accumbens Using Optogenetics

Optogenetic Stimulation of the Central Amygdala Using Channelrhodopsin

Optical Manipulation of Perfused Mouse Heart Expressing Channelrhodopsin-2 in Rhythm Control

Chronic Optogenetic Stimulation in Freely-Moving Rodents.

Other Format:

Printed edition:

ISBN:

978-1-0716-0830-2

9781071608302

Access Restriction:

Restricted for use by site license.