Yeast Protocols / edited by Wei Xiao.

Format:

Book

Contributor:

Xiao, Wei, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2196

Methods in Molecular Biology, 1940-6029 ; 2196

Language:

English

Subjects (All):

Microbiology.

Genetics.

Genetics and Genomics.

Local Subjects:

Microbiology.

Genetics and Genomics.

Physical Description:

1 online resource (XI, 260 pages) : 55 illustrations, 44 illustrations in color.

Edition:

4th ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community. .

Contents:

Use of Yeast Plasmids - Transformation and Inheritance Assays

Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae

CRISPR Nickase-Mediated Base Editing in Yeast

Genomic Promoter Shuffling by Using Recyclable Cassettes

Study Essential Gene Functions by Plasmid Shuffling

Scarless Genomic Protein Labeling in Saccharomyces cerevisiae

High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae

Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections

Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe

Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map

Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly

Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE

Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering

A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9

YeastNucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA

Assays for Autophagy III: Observing Dynamic Protein Trafficking

Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification

High-Throughput Rapid Yeast Chronological Lifespan Assay

Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast

Monitoring 5'-end Resection at Site-specific Double-strand Breaks by Southern Blot Analysis. .

Other Format:

Printed edition:

ISBN:

978-1-0716-0868-5

9781071608685

Access Restriction:

Restricted for use by site license.