Arabidopsis Protocols / edited by Jose J. Sanchez-Serrano, Julio Salinas.

Format:

Book

Contributor:

Sánchez-Serrano, Jose J., Editor.

Salinas, Julio, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2200

Methods in Molecular Biology, 1940-6029 ; 2200

Language:

English

Subjects (All):

Botany.

Plant Science.

Local Subjects:

Plant Science.

Physical Description:

1 online resource (XII, 465 pages) : 16 illustrations, 15 illustrations in color.

Edition:

4th ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This fourth edition compiles the most recent methodologies developed to exploit the Arabidopsis genome. Chapters detail access to public resources, to genetic, cell biology, biochemical and physiological techniques, and sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Arabidopsis Protocols, Fourth Edition aims to ensure successful results in the further study of this vital field.

Contents:

Handling Arabidopsis and other Brassicaceae: Growth, Preservation of Seeds, Transformation and Genetic Crosses

Bioinformatic Tools in Arabidopsis Research

Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene

Non-sterile Grafting Methods for Arabidopsis

CRISPR/Cas9-based Genome Editing Toolbox for Arabidopsis thaliana

Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration

Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts

Photosynthetic and Heterotrophic Cell Suspension Cultures

Genome-wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals

Visualising and Measuring Single Locus Dynamics in Arabidopsis thaliana

Identification and Quantification of Small RNAs

Flow Cytometry and Sorting in Arabidopsis

Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development

Using Genetically Encoded Fluorescent Biosensors for Quantitative in vivo Imaging

Using Tripartite Split-sfGFP for the Study of Membrane Protein-Protein Interactions

High-pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-labeling

Atomic Force Microscopy to Study Cell Wall Mechanics in Plants

Identification and Characterization of Reproductive Mutations in Arabidopsis

Metabolomic Analysis of Natural Variation in Arabidopsis

Untargeted Metabolomics of Arabidopsis Stomatal Immunity

Biotin-based Proximity Labeling Of Protein Complexes in Planta

Phosphoproteomic Analysis of Plant Membranes

RNA-binding Protein Immunoprecipitation and High Throughput Sequencing. .

Other Format:

Printed edition:

ISBN:

978-1-0716-0880-7

9781071608807

Access Restriction:

Restricted for use by site license.