The TNF Superfamily : Methods and Protocols / edited by Jagadeesh Bayry.

Format:

Book

Contributor:

Bayry, Jagadeesh, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2248

Methods in Molecular Biology, 1940-6029 ; 2248

Language:

English

Subjects (All):

Proteins.

Protein Biochemistry.

Local Subjects:

Protein Biochemistry.

Physical Description:

1 online resource (XIV, 284 pages) : 56 illustrations, 42 illustrations in color.

Edition:

2nd ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This second edition focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Chapters detail protocols on the signaling process of TNF family members, technical examples to investigating the role of TNF family members in physiopathologies, modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, methodologies for various assays of TNF family members and the production of recombinant molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, The TNF Superfamily: Methods and Applications, Second Edition serves to aid researchers investigating this key family of proteins.

Contents:

Signaling in TNFSF15-mediated Suppression of VEGF Production in Endothelial Cells

Sensitization of Airway Epithelial Cells to Toxin-Induced Death by TNF Superfamily Cytokines

Inhibition of Chondroitin Sulfate Proteoglycans by APRIL

Induction of Antigen-independent Proliferation of Regulatory T-cells by TNF superfamily ligands OX40L and GITRL

Transition from TNF-induced Inflammation to Death Signaling

Analysis of FcgR-Dependent Agonism Of Antibodies Specific for Receptors of the Tumor Necrosis Factor (TNF) Receptor Superfamily (TNFRSF)

Generation and Evaluation of Bispecific anti-TNF Antibodies Based on Single-Chain VHH Domains

In Vitro Physical and Functional Interaction Assays to Examine the Binding of Progranulin Derivative Atsttrin to TNFR2 and its anti-TNFα activity

Fluorescence Based TNFR1 Biosensor for Monitoring Receptor Structural and Conformational Dynamics and Discovery of Small Molecule Modulators

Production of Multi-Subtype Influenza Virus-Like Particles by Molecular Fusion with BAFF or APRIL for Vaccine Development

Experimental Methods for the Immunological Characterization of Paradoxical Psoriasis Reactions Induced by TNF-α Biologics

Methods for the Administration of EDAR Pathway Modulators in Mice

Analysis of Ligand-Receptor Interactions Using Bioluminescent TNF Superfamily (TNFSF) Ligand Fusion Proteins

Monitoring Atsttrin-mediated Inhibition of TNFα/NF-κβ Activation through in vivo Bioluminescence Imaging

The use of Murine Infection Models to Investigate the Protective Role of TNF in Central Nervous System Tuberculosis

Identification of CD137- and CD137L-expressing Cells in EL-4 Tumor

-Expression Profiling of Tumor Necrosis Factor Superfamily Ligands mRNA in Healthy and Injured Murine Kidneys

Analysis of Lymphotoxin Alpha Expression in Human Retina and Generation of Expression Vectors to Functional Characterization of Polymorphisms in the Tumor Necrosis Factor Locus

Detection of TNF-α Protein in Extracellular Vesicles Derived from Tumor Cells by Western Blotting

Expression of TNFRs by B and T lymphocytes in Tumor-Draining Lymph Nodes

Methods for Evaluation of TNF-α Inhibition Effect.

Other Format:

Printed edition:

ISBN:

978-1-0716-1130-2

9781071611302

Access Restriction:

Restricted for use by site license.