RNA Remodeling Proteins : Methods and Protocols / edited by Marc Boudvillain.

Format:

Book

Contributor:

Boudvillain, Marc, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2209

Methods in Molecular Biology, 1940-6029 ; 2209

Language:

English

Subjects (All):

Proteins.

Protein Biochemistry.

Local Subjects:

Protein Biochemistry.

Physical Description:

1 online resource (XIV, 437 pages) : 77 illustrations, 57 illustrations in color.

Edition:

2nd ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume provides the most current methods to study RNA remodeling proteins. Chapters detail methods, ranging from basic to complex, procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Remodeling Proteins: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Contents:

Key Points to Consider when Studying RNA Remodeling by Proteins

Happy Birthday : 30 Years of RNA Helicases

Known Inhibitors of RNA Helicases and their Therapeutic Potential

Identifying RNA Helicase Inhibitors Using Duplex Unwinding Assays

Thermal Shift Assay for Characterizing the Stability of RNA Helicases and their Interaction with Ligands

SRCD and FTIR Spectroscopies to Monitor Protein-induced Nucleic Acid Remodeling

High throughput Protein-Nucleic Acid Interaction Assay Based on Protein Induced Fluorescence Enhancement

Probing RNA Helicase Conformational Changes by Single-molecule FRET Microscopy

A Fluorescent Assay to Monitor Ligand-Dependent Closure of the Hexameric Rho Helicase Ring

A Simple Fluorescence Microplate Assay to Monitor RNA-DNA Hybrid Unwinding by the Bacterial Transcription Termination Factor Rho

Monitoring Enzymatic RNA G-quadruplex Unwinding Activities by Nuclease Sensitivity and Reverse Transcription Stop Assays

Using Magnetic Tweezers to Unravel the Mechanism of the G-quadruplex Binding and Unwinding Activities of DHX36 Helicase

Characterization of the Brr2 RNA Helicase and its Regulation by other Spliceosomal Proteins using Gel-based U4/U6 di-snRNA Binding and Unwinding Assays

Monitoring Acetylation of the RNA-helicase DDX3X, a Protein Critical for Formation of Stress Granules

In vivo cross-linking Followed by polyA Enrichment to Identify Yeast mRNA Binding Proteins

Deciphering the Dynamic Landscape of Transcription-associated mRNP quality Control Components over the Whole Yeast Genome

Probing the Conformational State of mRNPs using smFISH and SIM

Probing Transcriptome-wide RNA Structural Changes Dependent on the DEAD-box Helicase Dbp2

In situ hybridization - Proximity Ligation Assay (ISH-PLA) to Study the Interaction of HIV-1 RNA and Remodeling Proteins

RNAi Screening to Identify Factors that Control Circular RNA Localization

A Cell-Free System for Investigating Human MARF1 Endonuclease Activity

Monitoring eIF4E-dependent Nuclear 3' end mRNA Cleavage

An In Vitro Approach To Study RNase III Activities Of Plant RTL Proteins

Analysis of Bacillus subtilisRibonuclease Activity In Vivo

Assay of Bacillus subtilis Ribonuclease Activity in vitro

Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome. .

Other Format:

Printed edition:

ISBN:

978-1-0716-0935-4

9781071609354

Access Restriction:

Restricted for use by site license.