Shotgun Proteomics : Methods and Protocols / edited by Mónica Carrera, Jesús Mateos.

Format:

Book

Contributor:

Carrera, Mónica, Editor.

Mateos, Jesús, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2259

Methods in Molecular Biology, 1940-6029 ; 2259

Language:

English

Subjects (All):

Proteins.

Protein Biochemistry.

Local Subjects:

Protein Biochemistry.

Physical Description:

1 online resource (XVII, 324 pages) : 59 illustrations, 49 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This edition details a collection of specific shotgun proteomics-based laboratory techniques and applications developed in leading laboratories and proteomics units worldwide. Chapters cover a broad range of topics covering, shotgun proteomics of extracellular vesicles and subcellular structures, shotgun proteomics in non-model organisms, clinical proteomics, food proteomics, analysis of post-translational modifications and protein complexes, and data processing and storage. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Shotgun Proteomics: Methods and Protocols aims to be an up-to-date guide for researchers seeking to understand the proteome of any given biological sample.

Contents:

Mesenchymal Stem Cell - Derived Extracellular Vesicles Isolation and their Protein Cargo Characterisation

Clinical Proteomics for the Analysis of Circulating Extracellular Vesicles

Utilization of Laser Capture Microdissection Coupled to Mass Spectrometry to Uncover the Proteome of Cellular Protrusions

Isolation of Apoplastic Fluid From Woody Plants Leaves: Grapevine and Coffee as a Case Study

Shotgun Proteomics for L3 and L4 Anisakis simplex Development Stages

A Primer and Guidelines for Shotgun Proteomic Analysis in Non-Model Organisms

SWATH-MS Protocols in Human Diseases

Serum Proteomic Profiling in Rheumatoid Arthritis by Antibody Suspension Bead Arrays

Human Blood Plasma Investigation Employing 2d Uplc-Udmse Data-Independent Acquisition Proteomics

Metaproteomics Analysis of Host-Microbiota Interfaces

Mass Spectrometry-Based Analysis of Mycobacterial Single-Colony Proteome

Proteogenomic Approach for Mycobacterium Tuberculosis Investigation

Shotgun Proteomics for Food Microorganism Detection

Shotgun Proteomics and Protein-Based Bioinformatics for the Characterization of Food-Derived Bioactive Peptides

FTSC-labeling Coupled with 2DE-LC-MS/MS Analysis of Complex Protein Mixtures for Identification and Relative Quantification of Tissue Carbonylome

Mass Spectrometry-Based Proteomics for Analysis of Hydrophilic Phosphopeptides

Rapid Shotgun Phosphoproteomics Analysis

System-wide Profiling Of Protein-Complexes Via Size Exclusion Chromatography-Mass Spectrometry (SEC-MS)

Qualitative and Quantitative Shotgun Proteomics Data Analysis from Data-Dependent Acquisition Mass Spectrometry

The jPOST Repository as a Public Data Repository for Shotgun Proteomics.

Other Format:

Printed edition:

ISBN:

978-1-0716-1178-4

9781071611784

Access Restriction:

Restricted for use by site license.