In Vitro Models for Stem Cell Therapy : Methods and Protocols / edited by Peggy Stock, Bruno Christ.

Format:

Book

Contributor:

Stock, Peggy, Editor.

Christ, Bruno, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2269

Methods in Molecular Biology, 1940-6029 ; 2269

Language:

English

Subjects (All):

Stem cells.

Immunology.

Stem Cell Biology.

Local Subjects:

Stem Cell Biology.

Immunology.

Physical Description:

1 online resource (XII, 284 pages) : 55 illustrations, 42 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume looks at in vitro disease models representing the respiratory, hepatobiliary, osteochondral, nervous, dermal, ocular, immune system, and pathological biological processes like tumorigenesis for stem cell research. The chapters in this book cover a range of diseases and application of various stem cells such as adult stem cells and iPS. Chapters also discuss new methods to characterize and manipulate stem cells with the aim to better understand and improve their biological performance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, In Vitro Models for Stem Cell Therapy: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this exciting field.

Contents:

Consistent Inclusion of Mesenchymal Stem Cells into In Vitro Tumor Models

Pluripotent Stem Cells for Cell Therapy

In Vitro Methods for the Study of Glioblastoma Stem-Like Cell Radiosensitivity

Bioimaging of Mesenchymal Stem Cells Spatial Distribution and Interactions with 3D In Vitro Tumor Spheroids

Investigation of the MSC Paracrine Effects on Alveolar-Capillary Barrier Integrity in the In Vitro Models of ARDS

In Vitro Methods to Evaluate the Effects of Mesenchymal Stem Cells on TGFβ1-induced Pulmonary Fibrosis

Study of Mesenchymal Stem Cell-Mediated Mitochondrial Transfer in In Vitro Models of Oxidant-Mediated Airway Epithelial and Smooth Muscle Cell Injury

Co-Culture of Peripheral Blood Mononuclear Cells and Endothelial Colony Forming Cells from Cord Blood of Preterm Born Babies

Ex Vivo Model of Spontaneous Neuroretinal Degeneration for Evaluating Stem Cells' Paracrine Properties

Ex Vivo Normothermic Hypoxic Rat Liver Perfusion Model: An Experimental Setting for Organ Recondition and Pharmacological Intervention

Co-Culture of Human Mesenchymal Stromal Cells and Primary Mouse Hepatocytes

A 3D Dynamic In Vitro Model of Inflammatory Tendon Disease

Generation of Epidermal Equivalents from Hair Follicle Melanocytes, Keratinocytes, and Dermal Fibroblasts

Using Gene Expression Music Algorithms (GEMusicA) for the Characterization of Human Stem Cells

Evaluation of Extracellular Vesicles from Adipose Tissue-Derived Mesenchymal Stem Cells in Primary Human Chondrocytes from Patients with Osteoarthritis

Generation of Neural Stem Cells from Pluripotent Stem Cells for Characterization of Early Neuronal Development

Engineered Tissues Made from Human iPSC-derived Schwann Cells for Investigating Peripheral Nerve Regeneration In Vitro

Modulation of a Stem Cell Gene: LGR4 Knockout in a Human Cell Line by CRISPR/Cas Method

In Vitro Tool: 3D Cell Culture of Human Adipose Tissue-Derived Mesenchymal Stromal Cells on Low Stiffness Silicone Scaffolds.

Other Format:

Printed edition:

ISBN:

978-1-0716-1225-5

9781071612255

Access Restriction:

Restricted for use by site license.