Metastasis : Methods and Protocols / edited by Ulrike S. Stein.

Format:

Book

Contributor:

Stein, Ulrike S., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2294

Methods in Molecular Biology, 1940-6029 ; 2294

Language:

English

Subjects (All):

Cancer.

Cancer Biology.

Local Subjects:

Cancer Biology.

Physical Description:

1 online resource (XII, 348 pages) : 72 illustrations, 60 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume provides the most recent developments and methodologies on metastatic process, formation, and detection. Chapters guide readers through functional metastasis in vitro assays, non-mouse and mice metastasis models, methods for imaging metastasis, analyzing the tumor microenvironment, senescence and inflammation with respect to metastasis, methods to investgate the premetastatic/ metastatic, detecting biomarkers in patient, and bioinformatics to simulate the metastatic process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metastasis: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Contents:

Cancer Cell Invasion and Metastasis in Zebrafish Models (Danio rerio)

The Chicken Egg Chorionallantois Membrane (CAM) model as an in vivo Method for the Investigation of the Invasion and Metastasis Cascade of Malignant Tumor Cells

In vitro 3D Models of Tuneable Stiffness

Patient-derived Xenografts from Solid Tumors (PDX) for Models of Metastasis

Preparation and Culture of Organotypic Hippocampal Slices for the Analysis of Brain Metastasis and Primary Brain Tumor Growth

Measuring Mechanical and Adhesive Properties of Single Cells Using an Atomic Force Microscope

Analysing the Roles of Rho GTPases in Cancer Cell Adhesion to Endothelial Cells under Flow Conditions

Probing Intravascular Adhesion and Extravasation Of Tumor Cells With Microfluidics

Real-time Cell Migration Monitoring to Analyze Drug Synergism in the Scratch Assay using the IncuCyte system

Measurement of Metabolites from Migrating Cells

Matrix Degradation Assay to Measure the Ability of Tumor Cells to Degrade Extracellular Matrix

Evaluation of Real-Time In Vitro Invasive Phenotypes

Detection of Tumor Cell-induced Platelet Aggregation and Granule Secretion

Fatty Acid Driven Polarization of Suppressive Bone Marrow-Derived Macrophages Including Metabolic and Functional Analysis

From Whole Blood to Isolated Pro-Metastasis Immune Cells: An Ex-Vivo Approach to Isolate and Manipulate Immune Cells Contributing To Tumor Metastasis

A Bioinformatic Pipeline to Identify Biomarkers for Metastasis Formation from RNA Sequencing Data

Barcoding Technology for Multiplexed Analysis of Metastatic Ability In Vivo

In Vivo Assessment of Metastatic Cell Potential in Prostate Cancer

Isolation of Nucleoli for Characterization of Nucleolar Contents to Uncover Clues to Metastatic Progression

Analyzing the Role of Proteases in Breast Cancer Progression and Metastasis using Primary Cells from Transgenic Oncomice

Nuclear Medicine Imaging Procedures in Oncology

From Operating Table to Laboratory Bench: The path Towards Metastasis Research from the Clinical Setting. .

Other Format:

Printed edition:

ISBN:

978-1-0716-1350-4

9781071613504

Access Restriction:

Restricted for use by site license.