Ribosome Profiling : Methods and Protocols / edited by Vyacheslav M. Labunskyy.

Format:

Book

Contributor:

Labunskyy, Vyacheslav M., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2252

Methods in Molecular Biology, 1940-6029 ; 2252

Language:

English

Subjects (All):

Biochemistry.

Proteins.

Bioinformatics.

Protein Biochemistry.

Local Subjects:

Biochemistry.

Protein Biochemistry.

Bioinformatics.

Physical Description:

1 online resource (XI, 348 pages) : 72 illustrations, 53 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This book provides a single-source reference on the current state of the ribosome profiling method by describing experimental protocols for the quantitative analysis of translation in a variety of model organisms. In addition, the volume presents a detailed overview of the existing software tools and includes detailed description of methods for statistical analysis, data processing, and visualization of ribosome profiling data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ribosome Profiling: Methods and Protocols aims to provide the type of standardized protocols that have previously been unavailable in an effort to bypass the major barriers to wide use of ribosome profiling-based approaches.

Contents:

Monitoring Bacterial Translation Rates Genome-Wide

Identification of Translation Start Sites in Bacterial Genomes

Genome-Wide Ribosome Profiling of the Plasmodium falciparum Intraerythrocytic Developmental Cycle

Performing Ribosome Profiling to Assess Translation in Vegetative and Meiotic Yeast Cells

Quantitative Comparisons of Translation Activity by Ribosome Profiling with Internal Standards

Genome-Wide Analysis of Translation in Replicatively Aged Yeast

Tissue-Specific Ribosome Profiling in Drosophila

Measuring Organ-Specific Translation Elongation Rate in Mice

Active Ribosome Profiling with RiboLace: From Bench to Data Analysis

Poly-A Tailing and Adaptor Ligation Methods for Ribo-Seq Library Construction

Choice of Ribonucleases for Ribosome Profiling Experiments

Codon Resolution Analysis of Ribosome Profiling Data

Assessing Ribosome Distribution Along Transcripts with Polarity Scores and Regression Slope Estimates

Unsupervised Bayesian Prediction of RNA Translation from Ribosome Profiling Data

uORF-seqr: A Machine Learning-Based Approach to the Identification of Upstream Open Reading Frames in Yeast

Genome-Wide Analysis of Actively Translated Open Reading Frames Using RiboTaper/ORFquant.

Other Format:

Printed edition:

ISBN:

978-1-0716-1150-0

9781071611500

Access Restriction:

Restricted for use by site license.