Human pluripotent stem cells : a practical guide / Uma Lakshmipathy [and three others].

Format:

Book

Author/Creator:

Lakshmipathy, Uma, author.

Language:

English

Subjects (All):

Multipotent stem cells.

Stem cells.

Physical Description:

1 online resource (178 pages) : illustrations (some color), tables

Place of Publication:

Hoboken, New Jersey : Wiley Blackwell, 2018.

Summary:

Comprehensive coverage of the entire induced pluripotent stem cell basic work flow Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential. Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms. * Includes helpful tips and tricks that are normally omitted from regular research papers * Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations * Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner * Written by experts with extensive experience in stem cell technologies Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.

Notes:

Includes bibliographical references at the end of each chapters and index.

Description based on print version record.

ISBN:

9781119394365

1119394368

9781119394341

1119394341

9781119394372

1119394376

OCLC:

1004957085