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The role of Interleukin-4 receptor signaling in neonatal adipocyte precursors / Tammy Ying.
- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Ying, Tammy, author.
- Language:
- English
- Subjects (All):
- Genetics.
- Cellular biology.
- Molecular biology.
- Cell and molecular biology--Penn dissertations.
- Penn dissertations--Cell and molecular biology.
- Local Subjects:
- Genetics.
- Cellular biology.
- Molecular biology.
- Cell and molecular biology--Penn dissertations.
- Penn dissertations--Cell and molecular biology.
- Physical Description:
- 1 online resource (131 pages)
- Contained In:
- Dissertations Abstracts International 83-08B.
- Place of Publication:
- [Philadelphia, Pennsylvania] : University of Pennsylvania ; Ann Arbor : ProQuest Dissertations & Theses, 2021.
- Language Note:
- English
- System Details:
- Mode of access: World Wide Web.
- Summary:
- Adipose tissue has multiple functions including defense against the cold through thermogenesis of brown and beige adipocytes. The type 2 immune cytokine Interleukin-4 (IL-4) can increase beige adipogenesis in mature rodents. However, developing animals use a distinct adipocyte precursor compartment for adipogenesis compared to adults. In this body of work, we analyze the effects of IL-4 on the adipose tissue of developing rodents. In rats, we found that exogenous IL-4 induced an acute increase in beige adipogenesis and a persistent decrease in overall adipogenesis in neonatal inguinal white adipose tissue (iWAT). We determined that DPP4+ progenitors and ICAM1+ preadipocytes were the populations expressing IL-4 receptor in neonatal mice. Isolating neonatal ICAM1+ preadipocytes for RNA-seq, we discovered no significant differentially regulated genes in IL-4RαKO compared to WT. After adipogenic differentiation of IL-4RαKO and WT ICAM1+ preadipocytes, we also found no changes in adipocyte gene expression. However, using neonatal WT ICAM1+ preadipocytes, we show that IL-4 pretreatment increases Ucp1 expression. We also identified that IL-4 directly interferes with the process of adipogenesis, decreasing adipocyte maturation. Overall, these findings describe the role of IL-4 in developing iWAT, identify the precursor population altered by IL-4 receptor signaling and characterize long term effects of IL-4 that may influence the later onset of obesity.
- Notes:
- Source: Dissertations Abstracts International, Volume: 83-08, Section: B.
- Advisors: Simmons, Rebecca A.; Committee members: Henao-Mejia, Jorge; Seale, Patrick; Stanescu, Diana; Oliver, Paula; Riley, James L.
- Department: Cell and Molecular Biology.
- Ph.D. University of Pennsylvania 2021.
- Local Notes:
- School code: 0175
- ISBN:
- 9798780646686
- Access Restriction:
- Restricted for use by site license.
- This item must not be sold to any third party vendors.
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