Total Chemical Synthesis of Proteins.

Format:

Book

Contributor:

Brik, Ashraf.

Dawson, Philip.

Liu, Lei, 1977- editor.

Wiley InterScience (Online service)

Alumni and Friends Memorial Book Fund.

Language:

English

Subjects (All):

Proteins--Synthesis.

Proteins.

Physical Description:

1 online resource (627 pages)

polychrome

Place of Publication:

Newark : John Wiley & Sons, Incorporated, 2021.

System Details:

text file

Contents:

Cover

Title Page

Copyright

Contents

Preface

Chapter 1 Characterization of Protein Molecules Prepared by Total Chemical Synthesis

1.1 Introduction

1.2 Chemical Protein Synthesis

1.3 Comments on Characterization of Synthetic Protein Molecules

1.3.1 Homogeneity

1.3.2 Amino Acid Sequence

1.3.3 Chemical Analogues

1.3.4 Limitations of SPPS

1.3.5 Folding as a Purification Step

1.4 Summary

References

Chapter 2 Automated Fast Flow Peptide Synthesis

2.1 Introduction

2.2 Results

2.2.1 Summary

2.2.1.1 Mechanical Principles

2.2.1.2 Chemical Principles

2.2.1.3 User Interface Principles

2.2.1.4 Data Analysis Method

2.2.1.5 Outcome

2.2.2 First-generation Automated Fast Flow Peptide Synthesis

2.2.2.1 Key Findings

2.2.2.2 Design of First-generation AFPS

2.2.2.3 Characterization of First-generation AFPS

2.2.3 Second-generation Automated Fast Flow Peptide Synthesis

2.2.3.1 Key Findings

2.2.3.2 Design of Second-generation AFPS

2.2.3.3 Characterization and Use of Second-generation AFPS

2.2.4 Third-generation Automated Fast Flow Peptide Synthesis

2.2.4.1 Key Findings

2.2.4.2 Design of Third-generation AFPS

2.2.4.3 Characterization of Third-generation AFPS

2.2.4.4 Reagent Stability Study

2.2.5 Fourth-generation Automated Fast Flow Peptide Synthesis

2.2.5.1 Key Findings

2.2.5.2 Effect of Solvent on Fast Flow Synthesis

2.2.5.3 Design and Characterization of Fourth-generation AFPS

2.3 Conclusions

Acknowledgments

Chapter 3 N, S- and N, Se-Acyl Transfer Devices in Protein Synthesis

3.1 Introduction

3.2 N, S- and N, Se-Acyl Transfer Devices: General Presentation, Reactivity and Statistical Overview of Their Utilization

3.2.1 General Presentation of N, S- and N, Se-Acyl Transfer Devices

3.2.2 Relative Reactivity of N, S- and N, Se-Acyl Transfer Devices

3.2.3 A Statistical Overview of the Synthetic Use of N, S- and N, Se-Acyl Transfer Devices for Protein Total Chemical Synthesis

3.3 Preparation of SEA/SeEAoff and SEAlide Peptides

3.3.1 Preparation of SEA and SeEA Peptides

3.3.2 Preparation of SEAlide Peptides

3.4 Redox-controlled Assembly of Biotinylated NK1 Domain of the Hepatocyte Growth Factor (HGF) Using SEA and SeEA Chemistries

3.5 The Total Chemical Synthesis of GM2-AP Using SEAlide-based Chemistry

3.6 Conclusion

Chapter 4 Chemical Synthesis of Proteins Through Native Chemical Ligation of Peptide Hydrazides

4.1 Introduction

4.2 Development of Peptide Hydrazide-based Native Chemical Ligation

4.2.1 Conversion of Peptide Hydrazide to Peptide Azide

4.2.2 Acyl Azide-based Solid-phase Peptide Synthesis

4.2.3 Acyl Azide-based Solution-phase Peptide Synthesis

4.2.4 The Transesterification of Acyl Azide

4.2.5 Development of Peptide Hydrazide-based Native Chemical Ligation

Notes:

Electronic reproduction. Hoboken, N.J. Available via World Wide Web.

Print version record.

4.3 Optimization of Peptide Hydrazide-based Native Chemical Ligation.

Local Notes:

Acquired for the Penn Libraries with assistance from the Alumni and Friends Memorial Book Fund.

Other Format:

Print version: Brik, Ashraf. Total Chemical Synthesis of Proteins.

ISBN:

9783527823567

3527823565

9783527823574

3527823573

Publisher Number:

99990865716

Access Restriction:

Restricted for use by site license.