Electron tomography : methods for three-dimensional visualization of structures in the cell / Joachim Frank, editor.

Format:

Book

Contributor:

Frank, J. (Joachim), 1940-

Language:

English

Subjects (All):

Cytology.

Transmission electron microscopy.

Three-dimensional imaging in biology.

Cells.

Microscopy, Electron, Transmission--methods.

Cellular Structures--ultrastructure.

Imaging, Three-Dimensional--methods.

Tomography--methods.

Medical Subjects:

Microscopy, Electron, Transmission--methods.

Cellular Structures--ultrastructure.

Imaging, Three-Dimensional--methods.

Tomography--methods.

Physical Description:

xiv, 455 pages : illustrations ; 24 cm

Edition:

Second edition.

Place of Publication:

New York : Springer, [2006?]

Summary:

Electron tomography has become a standard technique with applications in cell biology, structural biology, and materials science. This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series, with special emphasis on the problems presented by limitations of data collection in the transmission electron microscope. In addition to chapters that are applicable to 3D reconstruction in all fields of science, such as radiological imaging in medicine and electron tomography in materials science, Electron Tomography also focuses on specimen preparation and imaging unique to biological electron microscopy.

This extensively revised second edition updates key contributions on the mathematics of 3D reconstruction, and includes new topics such as automated tomography, frozen sectioning of cells, and the interpretation of density maps through methods of fitting, docking, denoising, and segmentation. Each chapter is a self-contained treatise by a world expert in the author's field of research, resulting in an indispensable resource and companion for laboratories that practice electron tomography or seek to implement electron tomography as a tool for visualization of cells and cell components.

Contents:

Introduction : Principles of electron tomography / Joachim Frank

Sample shrinkage and radiation damage of plastic sections / Pradeep K. Luther

Electron tomography of frozen-hydrated sections of cells and tissues / Michael Marko, Chyong-Ere Hsieh and Carmen A. Mannella

The electron microscope as a structure projector / Peter W. Hawkes

Cryptomography : low-dose automated tomography of frozen-hydrated specimens / Abraham J. Koster and Montserrat Barcena

Fiducial marker and hybrid alignment methods for single- and double-axis tomography / David N. Mastronarde

Markerless alignment in electron tomography / Sami S. Brandt

Algorithms for three-dimensional reconstruction from the imperfect projection data provided by electron microscopy / Jose-Maria Carazo [and others].

Weighted back-projection methods / Michael Radermacher

Reconstruction with orthogonal functions / Elmar Zeitler

Resolution in electron tomography / Pawel A. Penczek and Joachim Frank

Denoising of electron tomograms / Reiner Hegerl and Achilleas S. Frangakis

Segmentation of three-dimensional electron tomographic images / Achilleas S. Frangakis and Reiner Hegerl

Segmentation of cell components using prior knowledge / Ming Jiang [and others]

Motif search in electron tomography / Achilleas S. Frangakis and Bimal K. Rath

Localization and classification of repetitive structures in electron tomograms of paracrystalline assemblies / Kenneth A. Taylor, Jun Liu and Hanspeter Winkler.

Notes:

Includes bibliographical references and index.

ISBN:

9780387312347

038731234X

OCLC:

70766144