Preclinical MRI of the Kidney : Methods and Protocols / edited by Andreas Pohlmann, Thoralf Niendorf.

Format:

Book

Author/Creator:

Pohlmann, Andreas., Editor.

Contributor:

Pohlmann, Andreas, Editor.

Niendorf, Thoralf, Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2216

Language:

English

Subjects (All):

Analytical chemistry.

Biology--Technique.

Biology.

Radiology.

Physiology.

Medicine--Research.

Medicine.

Biology--Research.

Analytical Chemistry.

Biological Techniques.

Biomedical Research.

Local Subjects:

Analytical Chemistry.

Biological Techniques.

Radiology.

Physiology.

Biomedical Research.

Physical Description:

1 online resource (XIV, 725 p. 202 illus., 169 illus. in color.)

Edition:

1st ed. 2021.

Place of Publication:

Springer Nature 2021

New York, NY : Springer US : Imprint: Humana, 2021.

Language Note:

English

Summary:

This Open Access volume provides readers with an open access protocol collection and wide-ranging recommendations for preclinical renal MRI used in translational research. The chapters in this book are interdisciplinary in nature and bridge the gaps between physics, physiology, and medicine. They are designed to enhance training in renal MRI sciences and improve the reproducibility of renal imaging research. Chapters provide guidance for exploring, using and developing small animal renal MRI in your laboratory as a unique tool for advanced in vivo phenotyping, diagnostic imaging, and research into potential new therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Preclinical MRI of the Kidney: Methods and Protocols is a valuable resource and will be of importance to anyone interested in the preclinical aspect of renal and cardiorenal diseases in the fields of physiology, nephrology, radiology, and cardiology. This publication is based upon work from COST Action PARENCHIMA, supported by European Cooperation in Science and Technology (COST). COST (www.cost.eu) is a funding agency for research and innovation networks. COST Actions help connect research initiatives across Europe and enable scientists to grow their ideas by sharing them with their peers. This boosts their research, career and innovation. PARENCHIMA (renalmri.org) is a community-driven Action in the COST program of the European Union, which unites more than 200 experts in renal MRI from 30 countries with the aim to improve the reproducibility and standardization of renal MRI biomarkers.

Contents:

Recommendations for Preclinical Renal MRI: A Comprehensive Open-Access Protocol Collection to Improve Training, Reproducibility, and Comparability of Studies

Animal Models of Renal Pathophysiology and Disease

Preparation and Monitoring of Small Animals in Renal MRI

Reversible (Patho-)Physiologically Relevant Test Interventions: Rationale and Examples

Preparation of Ex Vivo Rodent Phantoms for Developing, Testing, and Training MR Imaging of the Kidney and Other Organs

Quantitative Assessment of Renal Perfusion and Oxygenation by Invasive Probes: Basic Concepts

Ultrasound and Photoacoustic Imaging of the Kidney: Basic Concepts and Protocols

Hardware Considerations for Preclinical Magnetic Resonance of the Kidney

MRI Mapping of Renal T1: Basic Concept

MRI Mapping of the Blood Oxygenation Sensitive Parameter T2* in the Kidney: Basic Concept

Renal Diffusion Weighted Imaging (DWI) for Apparent Diffusion Coefficient (ADC), Intra Voxel Incoherent Motion (IVIM), and Diffusion Tensor Imaging (DTI): Basic Concept

Dynamic Contrast Enhancement (DCE)-MRI Derived Renal Perfusion and Filtration: Basic Concepts

Non-Invasive Renal Perfusion Measurement Using Arterial Spin Labelling (ASL) MRI: Basic Concept

Renal pH Imaging Using Chemical Exchange Saturation Transfer (CEST)-MRI: Basic Concepts

Sodium (23Na) MRI of the Kidney: Basic Concept

Hyperpolarized Carbon (13C) MRI of the Kidneys: Basic Concepts

Functional Imaging Using Fluorine (19F) MR Methods: Basic Concepts

MR Elastography of the Abdomen: Basic Concepts

Monitoring Renal Hemodynamics and Oxygenation by Invasive Probes: Experimental Protocol

Essential Practical Steps for MRI of the Kidney in Experimental Research

Assessment of Renal Volume with MRI: Experimental Protocol

Experimental Protocols for MRI Mapping of Renal T1

Experimental Protocols for MRI Mapping of the Blood Oxygenation Sensitive Parameters T2* and T2 in the Kidney

Renal MRI Diffusion: Experimental Protocol

Dynamic Contrast Enhanced (DCE)-MRI Derived Renal Perfusion and Filtration: Experimental Protocol

Renal Blood Flow Using Arterial Spin Labeling (ASL)-MRI: Experimental Protocol and Principles

Renal pH Mapping Using Chemical Exchange Saturation Transfer (CEST)-MRI: Experimental Protocol

Sodium (23Na) MRI of the Kidney: Experimental Protocol

Hyperpolarized Carbon (13C) MRI of the Kidney: Experimental Protocol

Fluorine (19F) MRI for Assessing Inflammatory Cells in the Kidney: Experimental Protocol

Fluorine (19F) MRI to Measure Renal Oxygen Tension and Blood Volume: Experimental Protocol

MR Elastography of the Abdomen: Experimental Protocols

Subsegmentation of the Kidney in Experimental MR Images Using Morphology-Based Regions-of-Interest or Multiple-Layer Concentric Objects

De-Noising for Improved Parametric MRIof the Kidney: Protocol for Non-Local Means Filtering

Analysis Protocols for MRI Mapping of Renal T1

Analysis Protocols for MRI Mapping of the Blood Oxygenation Sensitive Parameters T2* and T2 in the Kidney

Analysis of Renal Diffusion Weighted Imaging (DWI) Using Apparent Diffusion Coefficient (ADC) and Intra Voxel Incoherent Motion (IVIM) Models

Analysis Protocol for Dynamic Contrast Enhanced (DCE)-MRI of Renal Perfusion and Filtration

Quantitative Analysis of Renal Perfusion by Arterial Spin Labeling

Analysis Protocol for the Quantification of Renal pH Using Chemical Exchange Saturation Transfer (CEST)-MRI

Analysis Protocol for Renal Sodium (23Na) MR Imaging

Analysis Methods for Hyperpolarized Carbon (13C) MRI of the Kidney

Data Preparation Protocol for Low Signal-to-Noise Ratio Fluorine-19 MRI.

ISBN:

9781071609781

1071609785