Advanced Mass Spectrometry-Based Analytical Separation Techniques for Probing the Polar Metabolome / edited by Rawi Ramautar.

Format:

Book

Contributor:

Ramautar, Rawi, editor.

Series:

ISSO (Series)

Issn Series

Language:

English

Subjects (All):

Metabolites--Analysis.

Metabolites.

Mass spectrometry.

Physical Description:

1 online resource (352 pages)

Edition:

First edition.

Place of Publication:

London, England : The Royal Society of Chemistry, [2021]

Summary:

Discussing the state-of-the-art of the proposed topics in one single book for probing the polar metabolome, using relevant examples, is unique and needed in the metabolomics field.

Contents:

Intro

Title

Copyright

Contents

Chapter 1 State-of-the-art in LC-MS Approaches for Probing the Polar Metabolome

1.1 Introduction: Metabolomics and the Polar Metabolome

1.2 LC Technologies: General Introduction

1.3 LC Technologies to Probe the Polar Metabolome

1.3.1 Hydrophilic Interaction Liquid Chromatography (HILIC)

1.3.2 Reversed-phase Liquid Chromatography (RPLC) with Derivatization

1.3.3 Ion-pair Chromatography (IPC)

1.3.4 Ion Exchange Chromatography (IEC)

1.3.5 Mixed-mode Chromatography (MMC) and Multimode Chromatography

1.3.6 Two-dimensional Liquid Chromatography (2D-LC)

1.4 Conclusions

Abbreviations

References

Chapter 2 Chemical Derivatization for Polar Metabolome Analysis

2.1 Introduction

2.2 Chemical Derivatization LC-MS Analysis of Targeted Polar Metabolites

2.2.1 Amino Acid and Derivative Analysis

2.2.2 Nucleotide Analysis

2.2.3 Sugar Analysis

2.2.4 Small Organic Acid Analysis

2.3 Untargeted Analysis of Polar and Less-polar Metabolites

2.3.1 Main Features of CIL LC-MS

2.3.2 Data Processing in CIL LC-MS

2.3.3 Applications of CIL LC-MS

2.4 Concluding Remarks

Acknowledgements

Chapter 3 Ion Pair Liquid Chromatography-Mass Spectrometry for Probing the Polar Metabolome

3.1 Introduction

3.2 Ion Pair Liquid Chromatography (IP-LC)

3.2.1 Retention Mechanism of IP-LC

3.2.2 Critical Parameters of IP-LC

3.3 Mass Spectrometry

3.3.1 Targeted Analysis

3.3.2 Untargeted Analysis

3.4 Sample Preparation

3.4.1 Quenching

3.4.2 Extraction

3.4.3 Post-extraction

3.5 Ion Pair Liquid Chromatography-Mass Spectrometry-based Metabolomics

3.5.1 Central Carbon Metabolism

3.5.2 Amino Acids

3.5.3 Nucleotides

3.6 Potential Pitfalls in IP-LC-MS-based Metabolomics

3.6.1 Separation of Isomers

3.6.2 Ion Suppression.

3.6.3 Detrimental Effect of Metal Cations

3.6.4 System Contamination

Chapter 4 Hydrophilic Interaction Chromatography-Mass Spectrometry (HILIC-MS) Approaches for Probing the Polar Metabolome

4.1 HILIC-MS: A Powerful Analytical Approach for the Qualitative and Quantitative Analysis of the Polar Cellular Metabolome

4.2 From Stationary Phases to Analytical Conditions and Retention Mechanisms

4.2.1 Stationary Phases According to Chemical Structure

4.2.2 Gradient Elution, Buffers and Ionic Strength of Mobile Phases

4.2.3 What is Known About Retention Mechanisms in HILIC

4.3 Selectivity, Sensitivity and Reproducibility of HILIC-MS approaches

4.4 Applications: From Single-pathway Targeted Quantification to Multiple Pathway Targeted and Untargeted Profiling

4.5 Isotopic Profiling

4.6 Conclusive Remarks

Chapter 5 Advances in Solid Phase Microextraction (SPME) for Metabolomics

5.1 Introduction to Solid Phase Microextraction

5.2 Application of SPME in Foodomics

5.3 Application of SPME in Metabolomic Studies of Exposome

5.4 Metabolomics with SPME in Biomedical and Clinical Applications

5.5 Extraction Phases and SPME Devices for LC-based Metabolomics

5.6 Concluding Remarks

Chapter 6 State-of-the-art Capillary Electrophoresis Mass Spectrometry Methods for Analyzing the Polar Metabolome

6.1 Introduction

6.1.1 Capillary Electrophoresis Mass Spectrometry

6.1.2 Sample Injection Techniques

6.1.3 Capillary Electrophoresis Mass Spectrometry Interface Designs

6.1.4 Mass Spectrometer Selection

6.2 Recent Advances in CE-MS Profiling of the Polar Metabolome

6.2.1 Biofluids

6.2.2 Cell and Tissue Extracts

6.3 CE-MS Profiling of the Polar Metabolome from Minute Sample Amounts.

6.3.1 Minute Biofluids, Tissue, and Cells

6.3.2 Single-cell Capillary Electrophoresis Mass Spectrometry

6.3.3 Surface Sampling Capillary Electrophoresis Mass Spectrometry

6.4 Perspective and Future Outlook

Chapter 7 Ion Mobility-Time-of-flight Mass Spectrometry and Applications for Metabolomics

7.1 Introduction

7.1.1 Fundamentals: Gas Phase Mobility and Separation Principals

7.2 Instrumentation and Data Acquisition

7.2.1 DTIM-QTOFMS

7.2.2 TWIM-QTOFMS

7.2.3 TIMS-QTOFMS

7.3 Data Processing

7.4 Applications in Metabolomics

7.5 Discussion and Outlook

7.6 Conclusions

Chapter 8 NMR Approaches for Probing the Polar Metabolome

8.1 General Metabolomics Aspects

8.2 Sample Preparation

8.2.1 Quenching

8.2.2 Protein Removal

8.2.3 Fine Tuning the Polar Metabolome with Extraction Protocols

8.3 Optimizing Spectra Acquisition and Processing for Polar Metabolomics

8.3.1 Spectra Acquisition

8.3.2 Spectra Processing

8.3.3 Spectral Resolution Associated to Magnetic Field Strength and Probe Specificity

8.3.4 In vivo NMR Spectroscopy

8.4 Strategies for the Identification of Polar Metabolites

8.4.1 Available Literature for Metabolite Identification in NMR-based Metabolomics

8.4.2 Available Databases for Metabolite Identification in NMR-based Metabolomics

Chapter 9 State-of-the-art Mass Spectrometry-based Approaches to Explore the Polar Metabolome: Alzheimer's Disease as a Case Study

9.1 The Complexity of the Human Metabolome

9.2 Analytical Approaches to Explore the Polar Metabolome

9.2.1 Gas Chromatography-Mass Spectrometry

9.2.2 Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry

9.2.3 Capillary Electrophoresis-Mass Spectrometry

9.2.4 Direct Mass Spectrometry.

9.3 The Polar Metabolome in Alzheimer's Disease

9.3.1 Application of GC-MS Metabolomics in Alzheimer's Disease Research

9.3.2 Application of HILIC-MS Metabolomics in Alzheimer's Disease Research

9.3.3 Application of CE-MS Metabolomics in Alzheimer's Disease Research

9.3.4 Application of Other Metabolomics Platforms to Explore the Polar Metabolome in Alzheimer's Disease

9.4 Concluding Remarks

Chapter 10 Analytical Techniques for Material-limited Metabolomics: Recent Developments and Applications

10.1 Introduction

10.2 Sampling and Sample Preparation

10.2.1 Sample Collection

10.2.2 Sample Preparation

10.3 Micro- and Nanoscale Separation Techniques

10.4 Applications

10.4.1 Single-cell Metabolomics

10.4.2 Biomass-limited Tissue Applications

10.5 Conclusions and Perspectives

Chapter 11 More with Less: Single-cell Metabolomics by Mass Spectrometry

11.1 Introduction

11.2 Sampling Strategies

11.2.1 Sampling Considerations

11.2.2 Microfluidics

11.2.3 FACS

11.2.4 Micro-sampling

11.2.5 Discussion

11.3 Analytical Methods

11.3.1 Live Single-cell Mass Spectrometry (LSC-MS)

11.3.2 Single Probe and T-probe

11.3.3 Nano-LC

11.3.4 Manual Needle Microdissection and CE

11.3.5 (Nano-)DESI

11.3.6 LAESI

11.3.7 LDIDD

11.4 Data Considerations

11.5 Discussion

Chapter 12 Chiral Metabolomics

12.1 Introduction

12.2 Direct Chiral Metabolite Analysis by HPLC

12.2.1 Chiral Stationary Phases for Direct Analysis

12.2.2 Tagging

12.2.3 Direct 1D-LC versus 2D-LC

12.2.4 Metabolites Analyzed by a Direct Approach

12.3 Indirect Chiral Analysis by HPLC

12.3.1 Chiral Derivatization Agents

12.3.2 Opportunities and Caveats of the Indirect Approach with CDAs

12.3.3 Metabolites Analyzed by Indirect Analysis

12.4 Workflows.

12.4.1 Targeted Assays

12.4.2 Untargeted Assays

12.5 Conclusions

Subject Index.

Notes:

Description based on publisher supplied metadata and other sources.

Description based on print version record.

Includes bibliographical references.

ISBN:

9781839163524

1839163526

9781839163531

1839163534

OCLC:

1261364408