Cellular and molecular approaches in fish biology / edited by Ignacio Fernández Monzón and Jorge M.O. Fernandes.

Format:

Book

Contributor:

Fernández Monzón, Ignacio.

Fernandes, Jorge M. O.

Language:

English

Subjects (All):

Fishes--Cytology.

Fishes.

Fishes--Molecular aspects.

Marine biology.

Genre:

Electronic books.

Physical Description:

1 online resource

Place of Publication:

London : Academic Press, 2022.

System Details:

text file

Contents:

Front Cover

Cellular and Molecular Approaches in Fish Biology

Copyright

Contents

Contributors

Editor's biographies

Preface

Chapter 1 Technologies and strategies for ex situ conservation of aquatic organisms: The role of cryopreservation in long- ...

1 Factors and steps to consider in setting up a cryopreservation protocol

1.1 Cryopreservation definition

1.2 Vitrification

1.3 Setting up a cryopreservation protocol

1.4 Assay of freezing-thawing process

1.4.1 Cell integrity and functionality

1.4.2 Mitochondria and sperm motility

1.4.3 DNA damage analysis

1.5 Cell types used for genome cryobanking

2 Applications of cryopreservation techniques in aquatic animals

2.1 Historic aspects and current situation

2.1.1 Fish

2.1.2 Invertebrates

2.2 Cryopreservation applications: The use of cryobanks

3 Cryopreservation of different biological materials to create Genome Resource Banks

3.1 Sperm

3.2 Germline stem cells (GSCs)

3.3 Embryos and larvae cryopreservation

4 Examples of sperm cryopreservation in specific species

4.1 Endangered species

4.1.1 The European eel case study

4.2 Aquacultured species

4.2.1 The salmonid species case study

4.3 Model species

5 Conclusions and future perspectives

Acknowledgments

References

Further reading

Chapter 2 Molecular approaches on DNA damage evaluation after primordial germ cell cryopreservation in zebrafish

1 Introduction

2 Conventional DNA evaluation methods

3 DNA lesion detection by qPCR: A novel approach

4 Our contribution to DNA damage evaluation in cryopreserved germ cells

5 Specific protocol for DNA damage evaluation in zebrafish PGCs after cryopreservation

5.1 PGC isolation

5.2 Experimental groups

5.2.1 PGC cryopreservation

5.2.2 Without cryoprotectant treatment

5.2.3 Hydrogen peroxide treatment

5.2.4 Fresh samples

5.3 DNA isolation

5.4 Primer design

5.5 qPCR assay

5.6 Lesion rate calculation

6 Summary and future perspectives

Chapter 3 The role of epigenetics in fish biology and reproduction: An insight into the methods applied to aquaculture

1 Introduction: Concept and importance of epigenetics in terms of evolution

2 Epigenetic mechanisms

2.1 DNA covalent modifications

2.2 Histone modifications

2.3 Noncoding RNAs

3 The role of epigenetics in farmed fish species

3.1 Domestication

3.2 Nutrition

3.3 Reproduction

3.4 Rearing temperature

4 Epigenetic mechanisms in model species: A case study in zebrafish

4.1 The epigenetic events involved in gametogenesis

4.2 Epigenetic changes during embryo development

5 Techniques applied for the evaluation of DNA methylation

5.1 Evaluation of global DNA methylation

5.1.1 Luminometric methylation assay (LUMA): A brief overview

5.2 Analysis of gene promoter methylation

Other Format:

ebook version :

ISBN:

9780128222744

0128222743

OCLC:

1287200177

Access Restriction:

Restricted for use by site license.