Conformational changes in formylglycine-generating enzyme during the catalytic cycle : role of reducing agent and calcium / submitted by Md. Sarfaraz Alam.

Format:

Book

Author/Creator:

Alam, Sarfaraz, author.

Language:

English

Subjects (All):

Sulfatases.

Physical Description:

1 online resource (159 pages) : illustrations (some color), tables

Edition:

1. Auflage.

Place of Publication:

Gottingen, [Germany] : Cuvillier Verlag, 2015.

Summary:

Formylglycine generation, a unique process necessary for the activation of sulfatases, is a cotranslational event conserved from pro- to eukaryotes and is defined by the modification of the cysteine residue in the CxPxR motif of largely unfolded sulfatase polypeptides to C?-formylglycine (FGly). This process is confined to the endoplasmic reticulum (ER) in eukaryotes and catalyzed by the formylglycine-generating enzyme (FGE), a resident protein of the ER. Mutations in FGE, that impair FGly generation, lead to production of inactive sulfatases that manifests into an inherited metabolic disorder termed multiple sulfatase deficiency (MSD) in humans. Although FGE has been proposed to function as a cofactor independent monooxygenase, the precise mechanism is not yet clear. In the present study, a baculovirus-based method for expression and purification of recombinant FGE from cultured insect cells was established and the recombinant FGE was characterized in more detail.

Notes:

Includes bibliographical references.

Description based on online resource; title from PDF title page (ebrary, viewed October 3, 2017).

ISBN:

9783736981102

3736981104