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Temporal analysis of neurogenesis and microglia changes after mild traumatic brain injury in the adult male c57bl/6j mouse / Lyles R Clark.
- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Clark, Lyles R., author.
- Language:
- English
- Subjects (All):
- Neurosciences.
- Physiology.
- Behavior.
- Convulsions & seizures.
- Epilepsy.
- Memory.
- Brain research.
- Neurogenesis.
- Mental depression.
- Nervous system.
- Anxieties.
- Consciousness.
- Learning.
- Rodents.
- Executive function.
- Cell cycle.
- Traumatic brain injury.
- Neuroscience--Penn dissertations.
- Penn dissertations--Neuroscience.
- Local Subjects:
- Neurosciences.
- Physiology.
- Behavior.
- Convulsions & seizures.
- Epilepsy.
- Memory.
- Brain research.
- Neurogenesis.
- Mental depression.
- Nervous system.
- Anxieties.
- Consciousness.
- Learning.
- Rodents.
- Executive function.
- Cell cycle.
- Traumatic brain injury.
- Neuroscience--Penn dissertations.
- Penn dissertations--Neuroscience.
- Genre:
- Academic theses.
- Physical Description:
- 1 online resource (224 pages)
- Contained In:
- Dissertations Abstracts International 83-03B.
- Place of Publication:
- [Philadelphia, Pennsylvania] : University of Pennsylvania ; Ann Arbor : ProQuest Dissertations & Theses, 2021.
- Language Note:
- English
- System Details:
- Mode of access: World Wide Web.
- text file
- Summary:
- Traumatic brain injury (TBI) affects millions of people every year. Injuries can have a range of severities from mild to severe, but all injuries have the potential to result in lasting cognitive deficits. The underlying cause of these deficits is not known, but many of the symptoms people experience is in hippocampal-dependent behavior. One cellular candidate for mediating this hippocampal dysfunction is adult neurogenesis-a process known to be 1) affected by other types of injury, 2) involved in dentate gyrus-dependent cognitive function, and 3) modulated by microglia-mediated inflammation. In this thesis, I investigated how a mild TBI (mTBI) impacts adult neurogenesis and microglia populations in the dentate gyrus. Adult male mice were given lateral fluid percussion injury (LFPI) or Sham surgery and injected with BrdU 3 days post-injury (dpi). Mice were sacrificed 3, 7, or 31 dpi, and brain tissue was stained for Ki67, DCX, BrdU, andIba1 to examine numbers of proliferating cells, immature neurons, surviving cells, and microglia, respectively. I found that after mild LFPI, there is an increase inKi67+ cells 3 dpi; DCX+ cells 7 dpi; and BrdU+ cells 31 dpi. There was also an increase in Iba1+ cells 3 dpi. Together, these data suggest a transient increase in proliferation and neurogenesis, leading to increased survival of newborn neurons in injured mice, and this neurogenic increase is accompanied by microglial activation at the short-term time point. These results provide essential spatiotemporal detail to the literature on injury-induced neurogenesis and identify a therapeutically relevant window for immune modulation.
- Notes:
- Source: Dissertations Abstracts International, Volume: 83-03, Section: B.
- Advisors: Eisch, Amelia J.; Committee members: Cohen, Akiva S.; Cullen, D. Kacy; Bennett, F. Chris; Santhakumar, Vijayalakshmi.
- Department: Neuroscience.
- Ph.D. University of Pennsylvania 2021.
- Local Notes:
- School code: 0175
- ISBN:
- 9798535570105
- Access Restriction:
- Restricted for use by site license.
- This item must not be sold to any third party vendors.
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